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Literature summary extracted from

  • Entsch, B.; Ballou, D.P.
    Purification, properties, and oxygen reactivity of p-hydroxybenzoate hydroxylase from Pseudomonas aeruginosa [published erratum appears in Biochim Biophys Acta 1990 Mar 29;1038(1):139] (1989), Biochim. Biophys. Acta, 999, 313-322.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.2 0.0109
-
4-hydroxybenzoate
-
Pseudomonas aeruginosa
1.14.13.2 0.0232
-
NADPH
-
Pseudomonas aeruginosa
1.14.13.2 0.0371
-
O2
-
Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.2 45000
-
2 * 45000, SDS-PAGE Pseudomonas aeruginosa
1.14.13.2 75000
-
gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.2
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.2 additional information
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Pseudomonas aeruginosa protocatechuate + NADP+ + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.2 dimer 2 * 45000, SDS-PAGE Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.2 25
-
pH 5.5-8.5, stable Pseudomonas aeruginosa
1.14.13.2 40
-
pH 8.0, stable Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.2 8 8.1 HEPES or Tris-SO42- buffer Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 FAD contains 1 mol of FAD per mol of enzyme Pseudomonas aeruginosa
1.14.13.2 NADPH
-
Pseudomonas aeruginosa