EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.13.2 | - |
Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.2 | 3-hydroxybenzoate | above 1 mM | Pseudomonas fluorescens | |
1.14.13.2 | 4-Aminobenzoate | above 1 mM | Pseudomonas fluorescens | |
1.14.13.2 | 4-Fluorobenzoate | above 1 mM | Pseudomonas fluorescens | |
1.14.13.2 | 4-hydroxybenzoate | above 1 mM | Pseudomonas fluorescens | |
1.14.13.2 | benzoate | above 1 mM | Pseudomonas fluorescens | |
1.14.13.2 | Fe2+ | - |
Pseudomonas fluorescens | |
1.14.13.2 | protocatechuate | above 1 mM | Pseudomonas fluorescens | |
1.14.13.2 | salicylate | above 1 mM | Pseudomonas fluorescens | |
1.14.13.2 | Zn2+ | 0.2 mM, reversed by 10 mM glutathione | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.2 | 0.0213 | - |
4-hydroxybenzoate | - |
Pseudomonas fluorescens | |
1.14.13.2 | 0.0227 | - |
NADPH | reaction with 4-hydroxybenzoate | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.2 | 78000 | - |
gel filtration | Pseudomonas fluorescens |
1.14.13.2 | 83600 | - |
low speed sedimentation without reaching equilibrium | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | Pseudomonas fluorescens | inducible enzyme | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.2 | Pseudomonas fluorescens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.2 | 2,4-dihydroxybenzoate + NADPH + O2 | 1.5% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O | - |
? | |
1.14.13.2 | 3-bromo-4-hydroxybenzoate + NADPH + O2 | 3.2% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | ? | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas fluorescens | protocatechuate + NADP+ + H2O | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | inducible enzyme | Pseudomonas fluorescens | ? | - |
? | |
1.14.13.2 | 4-toluate + NADPH + O2 | 0.29% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | ? | - |
? | |
1.14.13.2 | benzene sulfonate + NADPH + O2 | 0.34% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | ? | - |
? | |
1.14.13.2 | additional information | under anaerobic conditions, the enzyme can catalyze a reduction of FAD by NADPH provided that 4-hydroxybenzoate is present | Pseudomonas fluorescens | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.2 | 36.7 | - |
4-hydroxybenzoate | - |
Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.2 | FAD | contains approximately 0.8 mol FAD per mol of enzyme | Pseudomonas fluorescens | |
1.14.13.2 | NADPH | no activity with NADH | Pseudomonas fluorescens |