EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.1 | expression in Escherichia coli | Pseudomonas putida |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.13.1 | apoenzyme is crystallized by dialysis method, using ammonium sulfate as the precipitant | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.1 | K163E | site directed mutagenesis, Lys163 is involved in the NADH-binding site | Pseudomonas putida |
1.14.13.1 | K163G | site directed mutagenesis, Lys163 is involved in the NADH-binding site | Pseudomonas putida |
1.14.13.1 | K163R | site directed mutagenesis, Lys163 is involved in the NADH-binding site | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.1 | additional information | - |
additional information | FAD, salicylate and NADH, comparison of wild-type, recombinant and mutant enzyme | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.1 | 45000 | - |
recombinant native and mutant protein, expressed in E. coli, SDS-PAGE | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.1 | Pseudomonas putida | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.1 | salicylate + NADH + H+ + O2 | - |
Pseudomonas putida | catechol + NAD+ + H2O + CO2 | - |
? |