Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Suzuki, K.; Gomi, T.; Kaidoh, T.; Itagaki, E.
    Hydroxylation of o-halogenophenol and o-nitrophenol by salicylate hydroxylase (1991), J. Biochem., 109, 348-353.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.1 additional information
-
additional information
-
Pseudomonas putida
1.14.13.1 0.0016
-
salicylate cosubstrate NADH Pseudomonas putida
1.14.13.1 0.034
-
o-Nitrophenol
-
Pseudomonas putida
1.14.13.1 0.1
-
NADPH cosubstrates O2 and salicylate Pseudomonas putida
1.14.13.1 0.13
-
O2 cosubstrate o-nitrophenol Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 additional information enzyme catalyzes formation of catechol from substrate analogues such as o-nitro-, o-amino-, o-iodo-, o-bromo- and o-chlorophenol by removing the ortho substituted groups Pseudomonas putida ?
-
?
1.14.13.1 additional information enzyme catalyses hydroxylation and dehalogenation of o-halogenophenols and also denitrification of o-nitrophenol with unusual stoichiometry Pseudomonas putida ?
-
?
1.14.13.1 additional information o-fluorophenol is not converted to catechol, though NADH oxidation is observed Pseudomonas putida ?
-
?
1.14.13.1 o-iodophenol + NADH + O2
-
Pseudomonas putida catechol + iodide + NAD+
-
?
1.14.13.1 o-nitrophenol + NADH + O2
-
Pseudomonas putida catechol + nitrite + NAD+
-
?
1.14.13.1 salicylate + NADH + H+ + O2
-
Pseudomonas putida catechol + NAD+ + H2O + CO2
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.1 8
-
substrate o-iodophenol Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 NADH
-
Pseudomonas putida