Literature summary extracted from

Chua, C.H.; Feng, Y.; Yeo, C.C.; Khoo, H.E.; Poh, C.L.
Identification of amino acid residues essential for catalytic activity of gentisate 1,2-dioxygenase from Pseudomonas alcaligenes NCIB 9867 (2001), FEMS Microbiol. Lett., 204, 141-146.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.13.11.4	-	Pseudomonas alcaligenes

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.13.11.4	H108D	no enzymatic activity	Pseudomonas alcaligenes
1.13.11.4	H110D	no enzymatic activity	Pseudomonas alcaligenes
1.13.11.4	H149D	no enzymatic activity	Pseudomonas alcaligenes
1.13.11.4	H151D	no enzymatic activity	Pseudomonas alcaligenes
1.13.11.4	additional information	double mutant Phe3Leu, Val334Ala, doubled enzymatic activity, mutants in conserved core regions with less activity	Pseudomonas alcaligenes

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.13.11.4	Pseudomonas alcaligenes	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.13.11.4	2,5-dihydroxybenzoate + O2	-	Pseudomonas alcaligenes	maleylpyruvate	-	?

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Release 2023.1 (January 2023)