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Literature summary extracted from

  • Matsushita, K.; Shinagawa, E.; Adachi, O.; Ameyama, M.
    Membrane-bound D-gluconate dehydrogenase from Pseudomonas aeruginosa. Purification and structure of cytochrome-binding form (1979), J. Biochem., 85, 1173-1181.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.99.3 cardiolipin stimulates in presence of Triton X-100 Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.3 membrane
-
 Pseudomonas aeruginosa 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.3 22000
-
 1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE Pseudomonas aeruginosa
1.1.99.3 50000
-
 1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE Pseudomonas aeruginosa
1.1.99.3 66000
-
 1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE Pseudomonas aeruginosa
1.1.99.3 124000 131000 sucrose density gradient centrifugation Pseudomonas aeruginosa
1.1.99.3 132000 138000 non-denaturing PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.3 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.3
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.3 561.5
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.3 D-gluconate + 2,6-dichlorophenolindophenol
-
 Pseudomonas aeruginosa 2-oxogluconate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.3 More in absence of Triton X-100 the enzyme becomes dimeric Pseudomonas aeruginosa
1.1.99.3 trimer 1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.3 cytochrome c1 contains a cytochrome c1, c-554(551), which might by a diheme cytochrome Pseudomonas aeruginosa
1.1.99.3 flavin contains covalently bound flavin Pseudomonas aeruginosa