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Literature summary extracted from
- Purification and characterization of D-sorbitol dehydrogenase from membrane of Gluconobacter suboxydans var. alpha (1982), Agric. Biol. Chem., 46, 135-141.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.99.21 | D-sorbitol stabilizes during solubilization and purification | Gluconobacter oxydans |
| 1.1.99.21 | ionic detergents, such as cetylpyridinium chloride, cetyltrimethyl ammonium bromide and sodium stearate, inactivate | Gluconobacter oxydans |
| 1.1.99.21 | Triton X-100, best detergent for solubilization | Gluconobacter oxydans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.21 | 30 | - |
D-sorbitol | - |
Gluconobacter oxydans |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.21 | cytoplasmic membrane | - |
Gluconobacter oxydans | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.21 | KCl | activation | Gluconobacter oxydans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.21 | 17000 | - |
1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE | Gluconobacter oxydans |
| 1.1.99.21 | 51000 | - |
1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE | Gluconobacter oxydans |
| 1.1.99.21 | 63000 | - |
1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE | Gluconobacter oxydans |
| 1.1.99.21 | 131000 | - |
calculated sum of each MW of the 3 subunits | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.21 | D-sorbitol + acceptor | Gluconobacter oxydans | high specificity | L-sorbose + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.21 | Gluconobacter oxydans | - |
var. alpha IFO 3254 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.21 | - |
Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.21 | 433 | - |
- |
Gluconobacter oxydans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.99.21 | 5°C, 50% loss of activity overnight | Gluconobacter oxydans |
| 1.1.99.21 | 5°C, storage for several months leads to cytochrome c decomposition | Gluconobacter oxydans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.21 | D-mannitol + acceptor | oxidation at 5% the rate of D-sorbitol | Gluconobacter oxydans | ? + reduced acceptor | - |
? | |
| 1.1.99.21 | D-sorbitol + acceptor | high specificity | Gluconobacter oxydans | L-sorbose + reduced acceptor | - |
? | |
| 1.1.99.21 | D-sorbitol + acceptor | high specificity, the following dyes act in vitro as acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, potassium ferricyanide, nitro blue tetrazolium or tetramethyl-p-phenylenediamine | Gluconobacter oxydans | L-sorbose + reduced acceptor | - |
? | |
| 1.1.99.21 | additional information | no oxidation of D-arabitol, L-iditol, meso-erythritol, galactitol, dulcitol, ribitol, xylitol | Gluconobacter oxydans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.21 | More | 1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.21 | 25 | - |
- |
Gluconobacter oxydans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.21 | 4.5 | - |
- |
Gluconobacter oxydans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.21 | cytochrome c | tightly bound , dehydrogenase-cytochrome c complex, separable by SDS-PAGE | Gluconobacter oxydans | |
| 1.1.99.21 | FAD | flavoprotein, covalently bound, 0.4 mol/mol enzyme | Gluconobacter oxydans | |
| 1.1.99.21 | additional information | NAD+, NADP+ or molecular oxygen are completely inactive | Gluconobacter oxydans |
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