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Literature summary extracted from
- Purification and characterization of polyethylene glycol dehydrogenase involved in the bacterial metabolism of polyethylene glycol (1980), Appl. Environ. Microbiol., 40, 701-705.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.20 | Cd2+ | - |
Flavobacterium sp. |  |
| 1.1.99.20 | Cd2+ | - |
Pseudomonas sp. | |
| 1.1.99.20 | Co2+ | - |
Flavobacterium sp. | |
| 1.1.99.20 | Co2+ | - |
Pseudomonas sp. | |
| 1.1.99.20 | Cu2+ | at 1 mM | Flavobacterium sp. | |
| 1.1.99.20 | Cu2+ | at 1 mM | Pseudomonas sp. | |
| 1.1.99.20 | Fe3+ | - |
Flavobacterium sp. | |
| 1.1.99.20 | Fe3+ | - |
Pseudomonas sp. | |
| 1.1.99.20 | Hg2+ | at 1 mM | Flavobacterium sp. | |
| 1.1.99.20 | Hg2+ | at 1 mM | Pseudomonas sp. | |
| 1.1.99.20 | Mn2+ | - |
Flavobacterium sp. | |
| 1.1.99.20 | Mn2+ | - |
Pseudomonas sp. | |
| 1.1.99.20 | monoiodoacetate | - |
Flavobacterium sp. | |
| 1.1.99.20 | monoiodoacetate | - |
Pseudomonas sp. | |
| 1.1.99.20 | Ni2+ | - |
Flavobacterium sp. | |
| 1.1.99.20 | Ni2+ | - |
Pseudomonas sp. | |
| 1.1.99.20 | p-chloromercuribenzoate | no inhibition with 2-mercaptoethanol | Flavobacterium sp. | |
| 1.1.99.20 | p-chloromercuribenzoate | no inhibition with 2-mercaptoethanol | Pseudomonas sp. | |
| 1.1.99.20 | Pb2+ | - |
Flavobacterium sp. | |
| 1.1.99.20 | Pb2+ | - |
Pseudomonas sp. | |
| 1.1.99.20 | Zn2+ | - |
Flavobacterium sp. | |
| 1.1.99.20 | Zn2+ | - |
Pseudomonas sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.20 | 3 | - |
PEG-6000 | - |
Pseudomonas sp. | |
| 1.1.99.20 | 3 | - |
PEG-6000 | - |
Flavobacterium sp. | |
| 1.1.99.20 | 10 | - |
Tetraethylene glycol | - |
Pseudomonas sp. | |
| 1.1.99.20 | 10 | - |
Tetraethylene glycol | - |
Flavobacterium sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.20 | additional information | no metal ion requirement | Pseudomonas sp. | |
| 1.1.99.20 | additional information | no metal ion requirement | Flavobacterium sp. | |
| 1.1.99.20 | potassium phosphate | - |
Pseudomonas sp. | |
| 1.1.99.20 | potassium phosphate | - |
Flavobacterium sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 60000 | - |
4 * 60000, mixed culture of Flavobacterium sp. and Pseudomonas sp., SDS-PAGE | Pseudomonas sp. |
| 1.1.99.20 | 60000 | - |
4 * 60000, mixed culture of Flavobacterium sp. and Pseudomonas sp., SDS-PAGE | Flavobacterium sp. |
| 1.1.99.20 | 240000 | - |
mixed culture of Flavobacterium sp. and Pseudomonas sp., gel filtration | Pseudomonas sp. |
| 1.1.99.20 | 240000 | - |
mixed culture of Flavobacterium sp. and Pseudomonas sp., gel filtration | Flavobacterium sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.20 | Flavobacterium sp. | - |
synergistic mixed culture with Pseudomonas sp. | - |
| 1.1.99.20 | Pseudomonas sp. | - |
synergistic mixed culture with Flavobacterium sp. | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.20 | mixed culture, using precipitation with ammonium sulfate, solubilization with laurylbetaine and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite and Sephadex G-200 | Pseudomonas sp. |
| 1.1.99.20 | mixed culture, using precipitation with ammonium sulfate, solubilization with laurylbetaine and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite and Sephadex G-200 | Flavobacterium sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 1.2 | - |
mixed culture | Pseudomonas sp. |
| 1.1.99.20 | 1.2 | - |
mixed culture | Flavobacterium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.20 | 1-heptanol + acceptor | - |
Pseudomonas sp. | n-heptanal + reduced acceptor | - |
? | |
| 1.1.99.20 | 1-heptanol + acceptor | - |
Flavobacterium sp. | n-heptanal + reduced acceptor | - |
? | |
| 1.1.99.20 | 1-hexanol + acceptor | - |
Pseudomonas sp. | n-hexanal + reduced acceptor | - |
? | |
| 1.1.99.20 | 1-hexanol + acceptor | - |
Flavobacterium sp. | n-hexanal + reduced acceptor | - |
? | |
| 1.1.99.20 | diols + acceptor | except ethylene glycol, 1,2-and 1,3-propenediol | Pseudomonas sp. | dialdehydes + reduced acceptor | - |
? | |
| 1.1.99.20 | diols + acceptor | except ethylene glycol, 1,2-and 1,3-propenediol | Flavobacterium sp. | dialdehydes + reduced acceptor | - |
? | |
| 1.1.99.20 | diols + acceptor | diethylene glycol and triethylene glycol are only poor substrates | Pseudomonas sp. | dialdehydes + reduced acceptor | - |
? | |
| 1.1.99.20 | diols + acceptor | diethylene glycol and triethylene glycol are only poor substrates | Flavobacterium sp. | dialdehydes + reduced acceptor | - |
? | |
| 1.1.99.20 | PEG-1000 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-1000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-20000 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-20000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-400 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-400 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-4000 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-4000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-6000 + acceptor | most active substrate of all PEGs | Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-6000 + acceptor | most active substrate of all PEGs | Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | polyethylene glycol + acceptor | first and main step in aerobic PEG-metabolism | Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | polyethylene glycol + acceptor | first and main step in aerobic PEG-metabolism | Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | primary alcohol + acceptor | ethanol is only a poor substrate | Pseudomonas sp. | aldehyde + reduced acceptor | - |
? | |
| 1.1.99.20 | primary alcohol + acceptor | ethanol is only a poor substrate | Flavobacterium sp. | aldehyde + reduced acceptor | - |
? | |
| 1.1.99.20 | primary alcohol + acceptor | methanol, glycerol, secondary alcohols and sugar alcohols are no substrates | Pseudomonas sp. | aldehyde + reduced acceptor | - |
? | |
| 1.1.99.20 | primary alcohol + acceptor | methanol, glycerol, secondary alcohols and sugar alcohols are no substrates | Flavobacterium sp. | aldehyde + reduced acceptor | - |
? | |
| 1.1.99.20 | primary alcohol + acceptor | 2,6-dichlorophenolindophenol is the acceptor in vitro | Pseudomonas sp. | aldehyde + reduced acceptor | - |
? | |
| 1.1.99.20 | primary alcohol + acceptor | 2,6-dichlorophenolindophenol is the acceptor in vitro | Flavobacterium sp. | aldehyde + reduced acceptor | - |
? | |
| 1.1.99.20 | tetraethylene glycol + acceptor | - |
Pseudomonas sp. | tetraethylene glycol aldehyde + reduced acceptor | - |
? | |
| 1.1.99.20 | tetraethylene glycol + acceptor | - |
Flavobacterium sp. | tetraethylene glycol aldehyde + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.20 | tetramer | 4 * 60000, mixed culture of Flavobacterium sp. and Pseudomonas sp., SDS-PAGE | Pseudomonas sp. |
| 1.1.99.20 | tetramer | 4 * 60000, mixed culture of Flavobacterium sp. and Pseudomonas sp., SDS-PAGE | Flavobacterium sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 60 | - |
- |
Pseudomonas sp. |
| 1.1.99.20 | 60 | - |
- |
Flavobacterium sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 35 | 70 | 66% of maximal activity at 35°C and 70°C | Pseudomonas sp. |
| 1.1.99.20 | 35 | 70 | 66% of maximal activity at 35°C and 70°C | Flavobacterium sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 35 | - |
stable below | Pseudomonas sp. |
| 1.1.99.20 | 35 | - |
stable below | Flavobacterium sp. |
| 1.1.99.20 | 60 | - |
94% loss of activity after 15 min at pH 8.0, the inicial velocity of 2,6-dichlorophenolindophenol reduction increases at temperatures up to around 60°C and rapidly declines at those above 60°C | Pseudomonas sp. |
| 1.1.99.20 | 60 | - |
94% loss of activity after 15 min at pH 8.0, the inicial velocity of 2,6-dichlorophenolindophenol reduction increases at temperatures up to around 60°C and rapidly declines at those above 60°C | Flavobacterium sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 8 | - |
toward PEG-6000 at a concentration of 5 mM | Pseudomonas sp. |
| 1.1.99.20 | 8 | - |
toward PEG-6000 at a concentration of 5 mM | Flavobacterium sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 7 | 9 | about half-maximal activity at pH 7.0 and 9.0 | Pseudomonas sp. |
| 1.1.99.20 | 7 | 9 | about half-maximal activity at pH 7.0 and 9.0 | Flavobacterium sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 7 | - |
40% loss of activity at 5°C after 12 h | Pseudomonas sp. |
| 1.1.99.20 | 7 | - |
40% loss of activity at 5°C after 12 h | Flavobacterium sp. |
| 1.1.99.20 | 7.5 | 9 | stable at 5°C for 12 h | Pseudomonas sp. |
| 1.1.99.20 | 7.5 | 9 | stable at 5°C for 12 h | Flavobacterium sp. |
| 1.1.99.20 | 10 | - |
40% loss of activity at 5°C after 12 h | Pseudomonas sp. |
| 1.1.99.20 | 10 | - |
40% loss of activity at 5°C after 12 h | Flavobacterium sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.20 | 2,6-dichlorophenolindophenol | electron acceptor | Pseudomonas sp. | |
| 1.1.99.20 | 2,6-dichlorophenolindophenol | electron acceptor | Flavobacterium sp. | |
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