EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.99.2 | concentrated by ultrafiltration and crystallized by vapor diffusion method | Alcaligenes sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.2 | (R)-2-hydroxyglutarate | - |
Alcaligenes sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.2 | 0.12 | - |
2-oxoglutarate | - |
Alcaligenes sp. | |
1.1.99.2 | 0.14 | - |
NADH | - |
Alcaligenes sp. | |
1.1.99.2 | 1.7 | - |
(S)-2-hydroxyglutarate | - |
Alcaligenes sp. | |
1.1.99.2 | 5.3 | - |
NAD+ | - |
Alcaligenes sp. | |
1.1.99.2 | 15 | - |
(R)-2-hydroxyglutarate | - |
Alcaligenes sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.2 | 42000 | - |
1 * 42000, SDS-PAGE | Alcaligenes sp. |
1.1.99.2 | 48000 | - |
gel filtration | Alcaligenes sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.2 | additional information | Alcaligenes sp. | high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.2 | Alcaligenes sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.2 | - |
Alcaligenes sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.99.2 | cell culture | high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration | Alcaligenes sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.2 | additional information | - |
- |
Alcaligenes sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.2 | (R)-2-hydroxyglutarate + NAD+ | about 10% of the activity with (S)-2-hydroxyglutarate | Alcaligenes sp. | 2-oxoglutarate + NADH | - |
ir | |
1.1.99.2 | (S)-2-hydroxyglutarate + NAD+ | in the reverse reaction almost 100% production of the S-isomer of 2-hydroxyglutarate | Alcaligenes sp. | 2-oxoglutarate + NADH + H+ | - |
r | |
1.1.99.2 | 2-hydroxy-n-hexanoate + NAD+ | at 12.4% of the activity with 2-oxoglutarate | Alcaligenes sp. | 2-oxo-n-hexanoate + NADH + H+ | - |
? | |
1.1.99.2 | 2-hydroxy-n-pentanoate + NAD+ | at 10.7% of the activity with 2-oxoglutarate | Alcaligenes sp. | 2-oxo-n-pentanoate + NADH + H+ | - |
? | |
1.1.99.2 | 2-hydroxybutanoate + NAD+ | at 9.6% of the activity with 2-oxoglutarate | Alcaligenes sp. | 2-oxobutanoate + NADH | - |
? | |
1.1.99.2 | 2-hydroxymalonate + NAD+ | at 29.3% of the activity with 2-oxoglutarate | Alcaligenes sp. | oxomalonate + NADH + H+ | - |
? | |
1.1.99.2 | additional information | high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration | Alcaligenes sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.2 | monomer | 1 * 42000, SDS-PAGE | Alcaligenes sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.2 | 7.2 | - |
reaction with alpha-oxoglutarate and NADH | Alcaligenes sp. |
1.1.99.2 | 11.5 | - |
reaction with L-2-hydroxyglutarate and NAD+ | Alcaligenes sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.2 | 5 | 8.5 | pH 5.0: about 45% of maximal activity, pH 8.5: 60% of maximal activity, reaction with alpha-oxoglutarate and NADH | Alcaligenes sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.2 | NAD+ | - |
Alcaligenes sp. | |
1.1.99.2 | NADH | - |
Alcaligenes sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.2 | 45 | - |
(R)-2-hydroxyglutarate | - |
Alcaligenes sp. |