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Literature summary extracted from

  • Suzuki, T.; Uozomi, T.; Beppu, T.
    Purification and characterization of a-hydroxyglutarate dehydrogenase from Alcaligenes sp. (1985), Agric. Biol. Chem., 49, 2939-2947.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.2 concentrated by ultrafiltration and crystallized by vapor diffusion method Alcaligenes sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.2 (R)-2-hydroxyglutarate
-
 Alcaligenes sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.2 0.12
-
 2-oxoglutarate
-
 Alcaligenes sp.
1.1.99.2 0.14
-
 NADH
-
 Alcaligenes sp.
1.1.99.2 1.7
-
 (S)-2-hydroxyglutarate
-
 Alcaligenes sp.
1.1.99.2 5.3
-
 NAD+
-
 Alcaligenes sp.
1.1.99.2 15
-
 (R)-2-hydroxyglutarate
-
 Alcaligenes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.2 42000
-
 1 * 42000, SDS-PAGE Alcaligenes sp.
1.1.99.2 48000
-
 gel filtration Alcaligenes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.2 additional information Alcaligenes sp. high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.2 Alcaligenes sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.2
-
 Alcaligenes sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.2 cell culture high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.2 additional information
-
-
 Alcaligenes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.2 (R)-2-hydroxyglutarate + NAD+ about 10% of the activity with (S)-2-hydroxyglutarate Alcaligenes sp. 2-oxoglutarate + NADH
-
 ir
1.1.99.2 (S)-2-hydroxyglutarate + NAD+ in the reverse reaction almost 100% production of the S-isomer of 2-hydroxyglutarate Alcaligenes sp. 2-oxoglutarate + NADH + H+
-
 r
1.1.99.2 2-hydroxy-n-hexanoate + NAD+ at 12.4% of the activity with 2-oxoglutarate Alcaligenes sp. 2-oxo-n-hexanoate + NADH + H+
-
 ?
1.1.99.2 2-hydroxy-n-pentanoate + NAD+ at 10.7% of the activity with 2-oxoglutarate Alcaligenes sp. 2-oxo-n-pentanoate + NADH + H+
-
 ?
1.1.99.2 2-hydroxybutanoate + NAD+ at 9.6% of the activity with 2-oxoglutarate Alcaligenes sp. 2-oxobutanoate + NADH
-
 ?
1.1.99.2 2-hydroxymalonate + NAD+ at 29.3% of the activity with 2-oxoglutarate Alcaligenes sp. oxomalonate + NADH + H+
-
 ?
1.1.99.2 additional information high levels of the enzyme is produced at the late stage of cultivation in the presence of citrate and with limited aeration Alcaligenes sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.2 monomer 1 * 42000, SDS-PAGE Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.2 7.2
-
 reaction with alpha-oxoglutarate and NADH Alcaligenes sp.
1.1.99.2 11.5
-
 reaction with L-2-hydroxyglutarate and NAD+ Alcaligenes sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.2 5 8.5 pH 5.0: about 45% of maximal activity, pH 8.5: 60% of maximal activity, reaction with alpha-oxoglutarate and NADH Alcaligenes sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.2 NAD+
-
 Alcaligenes sp.
1.1.99.2 NADH
-
 Alcaligenes sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.99.2 45
-
 (R)-2-hydroxyglutarate
-
 Alcaligenes sp.