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Literature summary extracted from

  • Nagasawa, T.; Mori, N.; Tani, Y.; Ogata, K.
    Characterization of choline dehydrogenase from Pseudomonas aeruginosa A-16 (1976), Agric. Biol. Chem., 40, 2077-2084.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.1 AgNO3 1 mM causes 100% inhibition Pseudomonas aeruginosa
1.1.99.1 Benzyltrimethylammonium chloride slight Pseudomonas aeruginosa
1.1.99.1 Betaine hydrochloride slight Pseudomonas aeruginosa
1.1.99.1 CuSO4 1 mM causes 100% inhibition Pseudomonas aeruginosa
1.1.99.1 dimethylglycine slight Pseudomonas aeruginosa
1.1.99.1 DL-Carnitine hydrochloride slight Pseudomonas aeruginosa
1.1.99.1 glycine slight Pseudomonas aeruginosa
1.1.99.1 HgCl2 1 mM causes 100% inhibition Pseudomonas aeruginosa
1.1.99.1 iodoacetic acid
-
 Pseudomonas aeruginosa
1.1.99.1 KCN 1 mM inhibits Pseudomonas aeruginosa
1.1.99.1 Mercuric acetate
-
 Pseudomonas aeruginosa
1.1.99.1 MnCl2 1 mM causes 100% inhibition Pseudomonas aeruginosa
1.1.99.1 p-chloromercuribenzoate
-
 Pseudomonas aeruginosa
1.1.99.1 Phenyltriethylammonium iodide slight Pseudomonas aeruginosa
1.1.99.1 Tetra-n-butylammonium iodide slight Pseudomonas aeruginosa
1.1.99.1 Tetra-n-propylammonium iodide slight Pseudomonas aeruginosa
1.1.99.1 Tetraethylammonium iodide slight Pseudomonas aeruginosa
1.1.99.1 Tetramethylammonium iodide slight Pseudomonas aeruginosa
1.1.99.1 Triethylbenzylammonium iodide slight Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.1 0.14
-
 phenazine methosulfate
-
 Pseudomonas aeruginosa
1.1.99.1 1.7
-
 choline
-
 Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.1 particle-bound
-
 Pseudomonas aeruginosa
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.1 Pseudomonas aeruginosa
-
 A-16 strain
-
1.1.99.1 Pseudomonas aeruginosa A-16
-
 A-16 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.1 partial, using Triton-treatment, chloroform-treatment and column chromatography on Sepharose 6B Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.1.99.1 -20°C , stable Pseudomonas aeruginosa
1.1.99.1 5°C, pH 7.4, complete loss of activity, partially purified enzyme, 5-6 days Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.1 choline + acceptor acceptor 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazolium bromide, particulate enzyme: 12.4% relative activity to phenazine methosulfate Pseudomonas aeruginosa betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor cytochrome c, particulate enzyme: 3.1% relative activity to phenazine methosulfate Pseudomonas aeruginosa betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor acceptor ferricyanide, particulate enzyme: 112% relative activity to phenazine methosulfate Pseudomonas aeruginosa betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor acceptor 2,6-dichlorophenolindophenol, particulate enzyme: 3.2% relative activity to phenazine methosulfate Pseudomonas aeruginosa betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor acceptor: phenazine methosulfate, 100% relative activity Pseudomonas aeruginosa betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor electron acceptor: phenazine methosulfate Pseudomonas aeruginosa betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor acceptor 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazolium bromide, particulate enzyme: 12.4% relative activity to phenazine methosulfate Pseudomonas aeruginosa A-16 betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor cytochrome c, particulate enzyme: 3.1% relative activity to phenazine methosulfate Pseudomonas aeruginosa A-16 betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor acceptor ferricyanide, particulate enzyme: 112% relative activity to phenazine methosulfate Pseudomonas aeruginosa A-16 betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor acceptor 2,6-dichlorophenolindophenol, particulate enzyme: 3.2% relative activity to phenazine methosulfate Pseudomonas aeruginosa A-16 betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor acceptor: phenazine methosulfate, 100% relative activity Pseudomonas aeruginosa A-16 betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + phenazine methosulfate
-
 Pseudomonas aeruginosa betaine aldehyde + reduced phenazine methosulfate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.1 35
-
 10 min, pH 8.3, 10% loss of activity Pseudomonas aeruginosa
1.1.99.1 40
-
 10 min, pH 8.3, 50% loss of activity Pseudomonas aeruginosa
1.1.99.1 50
-
 10 min, pH 8.3, 100% loss of activity Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.1 9
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.1 7.5 10 about 50% of activity maximum at pH 7.5 and pH 10.0 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.1 additional information the enzyme requires no readily dissociable coenzyme for activity Pseudomonas aeruginosa