EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.1 | coenzyme Q | primary electron acceptor in vivo | Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
1.1.99.1 | repeated freezing and thawing: rapid loss of activity | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.1 | 2-Dimethylaminoethanol | - |
Rattus norvegicus | |
1.1.99.1 | Betaine aldehyde | choline dehydrogenase activity | Rattus norvegicus | |
1.1.99.1 | DL-3-hydroxybutyrate | at 16.7 mM acts as weak inhibitor | Rattus norvegicus | |
1.1.99.1 | Monoethanolamine | - |
Rattus norvegicus | |
1.1.99.1 | Semicarbazide | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.1 | 1.1 | - |
phenazine methosulfate | with choline | Rattus norvegicus | |
1.1.99.1 | 3.1 | - |
Betaine aldehyde | at 1 mM phenazine methosulfate | Rattus norvegicus | |
1.1.99.1 | 7 | - |
choline | electron acceptor: phenazine methosulfate | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.1 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.1 | choline + coenzyme Q1 | Rattus norvegicus | mitochondrial oxidation of choline | betaine aldehyde + reduced coenzyme Q1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.1 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.1 | using column chromatography of the chemically modified enzyme with 5,5'-dithiobis(2-nitrobenzoic acid) on DEAE-Sepharose CL-6B and choline-Sepharose 4B with C3-spacer, subsequent release of the thionitrobenzoate with dithiothreitol, and a second column chromatography on DEAE-Sepharose CL-6B in the presence of 0.1% Triton X-100 | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.99.1 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.1 | 6.3 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.99.1 | -15°C, 50% loss of activity, 1 month | Rattus norvegicus |
1.1.99.1 | -20°C, stable, partially purified enzyme | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.1 | choline + acceptor | the enzyme can use only choline and betaine aldehyde | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
1.1.99.1 | choline + acceptor | absolute requirement for an electron acceptor other than O2 | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
1.1.99.1 | choline + acceptor | reverse reaction at 5.2% of forward reaction | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
1.1.99.1 | choline + acceptor | coenzyme Q, primary electron acceptor in vivo | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
1.1.99.1 | choline + acceptor | electron acceptor: phenazine methosulfate | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
1.1.99.1 | choline + acceptor | not: 2-dimethylaminoethanol, monoethanolamine | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
r | |
1.1.99.1 | choline + coenzyme Q1 | mitochondrial oxidation of choline | Rattus norvegicus | betaine aldehyde + reduced coenzyme Q1 | - |
? | |
1.1.99.1 | choline + phenazine methosulfate | - |
Rattus norvegicus | betaine aldehyde + reduced phenazine methosulfate | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.1 | 45 | - |
- |
Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.1 | 9 | - |
- |
Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.1 | coenzyme Q | primary electron acceptor in vivo | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.1 | 1 | - |
2-Dimethylaminoethanol | inhibits the enzyme competitively | Rattus norvegicus | |
1.1.99.1 | 3.3 | - |
Betaine aldehyde | inhibits the oxidation of choline competitively | Rattus norvegicus |