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Literature summary extracted from

  • Witt, S.; Singh, M.; Kalisz, H.M.
    Structural and kinetic properties of nonglycosylated recombinant Penicillium amagasakiense glucose oxidase expressed in Escherichia coli (1998), Appl. Environ. Microbiol., 64, 1405-1411.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.4 cloning of the gene encoding the enzyme and expression in Escherichia coli Penicillium amagasakiense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 5.7
-
 beta-D-glucose native enzyme Penicillium amagasakiense
1.1.3.4 6.2
-
 beta-D-glucose recombinant enzyme Penicillium amagasakiense
1.1.3.4 6.3
-
 beta-D-glucose deglycosylated enzyme Penicillium amagasakiense
1.1.3.4 8.3
-
 2-deoxy-D-glucose recombinant enzyme Penicillium amagasakiense
1.1.3.4 106
-
 D-mannose recombinant enzyme Penicillium amagasakiense
1.1.3.4 384
-
 D-xylose recombinant enzyme Penicillium amagasakiense
1.1.3.4 952
-
 D-galactose recombinant enzyme Penicillium amagasakiense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.4 60000
-
 2 * 60000, SDS-PAGE, recombinant enzyme Penicillium amagasakiense
1.1.3.4 120000
-
 recombinant enzyme, native PAGE Penicillium amagasakiense

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Penicillium amagasakiense
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.4 no glycoprotein recombinant enzyme is nonglycosylated Penicillium amagasakiense

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4 of the reactivated recombinant enzyme, using mild acidification and anion-exchange chromatography on Q-Sepharose Penicillium amagasakiense

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.3.4 refolding after treatment with 8 M urea and 30 mM dithiothreitol and subsequent dilution in a buffer containing reduced glutathione, FAD and glycerol Penicillium amagasakiense

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.4 additional information
-
-
 Penicillium amagasakiense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 2-deoxy-D-glucose + O2 + H2O recombinant enzyme Penicillium amagasakiense 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O glucose is the primary substrate, recombinant enzyme Penicillium amagasakiense D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 D-galactose + O2 + H2O recombinant enzyme Penicillium amagasakiense ? + H2O2
-
 ?
1.1.3.4 D-xylose + O2 + H2O recombinant enzyme Penicillium amagasakiense ?
-
 ?
1.1.3.4 mannose + O2 + H2O recombinant enzyme Penicillium amagasakiense ? + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.4 dimer 2 * 60000, SDS-PAGE, recombinant enzyme Penicillium amagasakiense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 28 40 recombinant enzyme Penicillium amagasakiense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.4 1950
-
 beta-D-glucose deglycosylated enzyme Penicillium amagasakiense
1.1.3.4 2000
-
 beta-D-glucose recombinant enzyme Penicillium amagasakiense
1.1.3.4 2000
-
 beta-D-glucose native enzyme Penicillium amagasakiense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.2 6.2 recombinant enzyme Penicillium amagasakiense

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.4 4.5 5.2 recombinant enzyme, exhibits more than 80% of the maximum activity Penicillium amagasakiense
1.1.3.4 5 7 recombinant enzyme, more than 90% of the residual activity retained after 72 h incubation at room temperature Penicillium amagasakiense
1.1.3.4 6.2 6.5 recombinant enzyme, exhibits more than 80% of the maximum activity Penicillium amagasakiense
1.1.3.4 7
-
 above, the recombinant enzyme is slightly less stable than native and deglycosylated enzyme Penicillium amagasakiense