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Literature summary extracted from

  • Shin, K.S.; Youn, H.D.; Han, Y.H.; Kang, S.O.; Hah, Y.C.
    Purification and characterization of D-glucose oxidase from white-rot fungus Pleurotus ostreatus (1993), Eur. J. Biochem., 215, 747-752.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.4 KCN substancial stimulation Pleurotus ostreatus
1.1.3.4 p-chloromercuribenzoate substancial stimulation Pleurotus ostreatus
1.1.3.4 Sodium nitrate substancial stimulation Pleurotus ostreatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.4 8-hydroxyquinoline
-
 Pleurotus ostreatus
1.1.3.4 Ag+ inhibits oxidation of the reduced FAD moiety competing with molecular oxygen as a hydrogen acceptor Pleurotus ostreatus
1.1.3.4 Ag2SO4
-
 Pleurotus ostreatus
1.1.3.4 HgCl2
-
 Pleurotus ostreatus
1.1.3.4 NaN3
-
 Pleurotus ostreatus
1.1.3.4 phenylmercuric acetate
-
 Pleurotus ostreatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 additional information
-
 additional information
-
 Pleurotus ostreatus
1.1.3.4 1.34
-
 beta-D-glucose
-
 Pleurotus ostreatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.4 periplasmic space
-
 Pleurotus ostreatus 42597
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.4 70000
-
 4 * 70000, SDS-PAGE Pleurotus ostreatus
1.1.3.4 290000
-
 gel filtration Pleurotus ostreatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.4 additional information Pleurotus ostreatus important role in lignin-degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Pleurotus ostreatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4 using ammonium sulfate precipitation, gel filtration, anion-exchange and hydrophobic-interaction chromatography Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 2-deoxy-D-glucose + O2 + H2O 38% relative activity to D-glucose Pleurotus ostreatus 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 alpha-methyl-D-glucoside + O2 + H2O 13% relative activity to D-glucose Pleurotus ostreatus ? + H2O2
-
 ?
1.1.3.4 beta-D-glucose + 2,6-dichlorophenol indophenol
-
 Pleurotus ostreatus D-glucono-1,5-lactone + ?
-
 ?
1.1.3.4 beta-D-glucose + 4-benzoquinone
-
 Pleurotus ostreatus D-glucono-1,5-lactone + 4-benzoquinol
-
 r
1.1.3.4 beta-D-glucose + N,N,N',N'-tetramethyl-1,4-phenylenediamine
-
 Pleurotus ostreatus ?
-
 r
1.1.3.4 beta-D-glucose + O2 + H2O 2,6-dichloroindophenol, N,N,N',N'-tetramethyl-1,4-phenylenediamine, and 4-benzoquinone can function as electron acceptors Pleurotus ostreatus D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O glucose is the primary substrate for the enzyme Pleurotus ostreatus D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 cellobiose + O2 + H2O 13% relative activity to D-glucose Pleurotus ostreatus ? + H2O2
-
 ?
1.1.3.4 D-glucono-1,5-lactone + O2 + H2O 80% relative activity to D-glucose Pleurotus ostreatus ? + H2O2
-
 ?
1.1.3.4 L-gulono-gamma-lactone + O2 + H2O 62% relative activity to D-glucose Pleurotus ostreatus ? + H2O2
-
 ?
1.1.3.4 L-sorbose + O2 + H2O 86% relative activity to D-glucose Pleurotus ostreatus ?
-
 ?
1.1.3.4 mannose + O2 + H2O 9% relative activity to D-glucose Pleurotus ostreatus ? + H2O2
-
 ?
1.1.3.4 additional information important role in lignin-degradation Pleurotus ostreatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.4 tetramer 4 * 70000, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 50
-
-
 Pleurotus ostreatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 70
-
 stable for 120 min Pleurotus ostreatus
1.1.3.4 73
-
 70% loss of activity after 60 min Pleurotus ostreatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.4 0.33
-
 N,N,N',N'-tetramethyl-1,4-phenylenediamine
-
 Pleurotus ostreatus
1.1.3.4 1.13
-
 2,6-dichloroindophenol
-
 Pleurotus ostreatus
1.1.3.4 15.5
-
 4-benzoquinone
-
 Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.5 6
-
 Pleurotus ostreatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.4 FAD 4 mol flavin per mol of enzyme Pleurotus ostreatus