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Literature summary extracted from

  • Pazur, J.H.
    Glucose oxidase from Aspergillus niger (1966), Methods Enzymol., 9, 82-87.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.3.4 3°C, little loss of activity, 3 years Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.4 150000 153000 sedimentation and diffusion data Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O Aspergillus niger
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.4 glycoprotein
-
 Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4 using dialysis, ammonium sulfate fractionation and column chromatography on DEAE-cellulose Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.4 mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.4 additional information
-
-
 Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.1.3.4 3°C, purified enzyme, several years Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 2-deoxy-D-glucose + O2 + H2O 20% relative activity to D-glucose Aspergillus niger 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 3-deoxy-D-glucose + O2 + H2O 1% relative activity to D-glucose Aspergillus niger 3-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 4-deoxy-D-glucose + O2 + H2O 2% relative activity to D-glucose Aspergillus niger 4-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 4-O-methy-D-glucose + O2 + H2O 15% relative activity to D-glucose Aspergillus niger 4-O-methyl-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 6-deoxy-D-glucose + O2 + H2O 10% relative activity to D-glucose Aspergillus niger 6-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 6-O-methyl-D-glucose + O2 + H2O 1% relative activity to D-glucose Aspergillus niger 6-O-methyl-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 alpha-D-glucose + O2 + H2O very slow reaction Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O highly specific Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O glucose is the primary substrate for the enzyme Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 mannose + O2 + H2O 1% relative activity to D-glucose Aspergillus niger ? + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 additional information
-
 the enzyme is very stable at cold temperatures Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.5
-
-
 Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.4 FAD 2 mol per mol of enzyme Aspergillus niger