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Literature summary extracted from

  • Nakamura, S.; Hayashi, S.; Koga, K.
    Effect of periodate oxidation on the structure and properties of glucose oxidase (1976), Biochim. Biophys. Acta, 445, 294-308.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.4 SDS, 1%, 30°C, pH 5.5, stable 30 h incubation Aspergillus niger
1.1.3.4 urea: 7 M, 5 min, activity fully restored Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 0.18
-
O2 periodate-oxidized enzyme Aspergillus niger
1.1.3.4 0.2
-
O2 native enzyme Aspergillus niger
1.1.3.4 26
-
D-glucose native enzyme Aspergillus niger
1.1.3.4 28
-
beta-D-glucose periodate-oxidized enzyme Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.4 150000 153000 periodate-oxidized enzyme Aspergillus niger
1.1.3.4 150000 153000 sedimentation and diffusion data Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O Aspergillus niger
-
D-glucono-1,5-lactone + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.4 glycoprotein native enzyme contains 12% carbohydrate by weight, main component: mannose, the carbohydrate moiety plays a role in increasing the stability of the protein moiety, but does not directly participate in the catalytic activity, in the immunological reactivity, or in maintaining the conformation of the enzyme protein, periodate treatment decreases the carbohydrate content to about 40% of its original value Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.4 commercial preparation
-
Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O
-
Aspergillus niger D-glucono-1,5-lactone + H2O2
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 72.4
-
denaturation point of native enzyme Aspergillus niger
1.1.3.4 72.8
-
denaturation point of periodate-oxidized enzyme Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.4 323
-
beta-D-glucose periodate-oxidized enzyme Aspergillus niger
1.1.3.4 337
-
beta-D-glucose native enzyme Aspergillus niger