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Literature summary extracted from

  • Demura, M.; Asakura, T.
    Immobilization of glucose oxidase with Bombyx mori silk fibroin by only stretching treatment and its application to glucose sensor (1989), Biotechnol. Bioeng., 33, 598-603.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 analysis enzyme immobilized in Bombyx mori silk fibroin membrane applied to glucose sensor Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 4
-
 beta-D-glucose immobilized enzyme, immobilized membrane with ratio stretching 1.25 Bombyx mori
1.1.3.4 4.5
-
 beta-D-glucose immobilized enzyme, methanol treated Bombyx mori
1.1.3.4 5.4
-
 beta-D-glucose immobilized enzyme, immobilized membrane with ratio stretching 3 Bombyx mori
1.1.3.4 25.2
-
 beta-D-glucose free enzyme Bombyx mori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O Bombyx mori
-
 D-glucono-1,5-lactone + H2O2 in a subsequent step D-glucono-1,5-lactone is nonenzymatically hydrolyzed to D-gluconic acid ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Bombyx mori
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.3.4 4°C, immobilized, membrane in dried state, more than 12 months Bombyx mori
1.1.3.4 4°C, more than 90% activity retained, 100 mM phosphate buffer, pH 7, immobilized, 4 months Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Bombyx mori D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Bombyx mori D-glucono-1,5-lactone + H2O2 in a subsequent step D-glucono-1,5-lactone is nonenzymatically hydrolyzed to D-gluconic acid ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 50
-
 the activity of the free enzyme decreases to 30% of the original value, the activity of the immobilized enzyme scarcely decreases Bombyx mori
1.1.3.4 70
-
 inmobilized enzyme, more than 60% of the activity remains Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.2 7 immobilized on silk fibroin Bombyx mori
1.1.3.4 5.7
-
 soluble enzyme Bombyx mori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.4 5 8
-
 Bombyx mori