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Literature summary extracted from

  • Ander, P.
    The cellobiose-oxidizing enzymes CBQ and CbO as related to lignin and cellulose degradation, a review (1994), FEMS Microbiol. Rev., 13, 297-312.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.18 cellobionolactone more than 0.59 mM inhibit CBO activity Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.0012
-
 cytochrome c
-
 Phanerodontia chrysosporium
1.1.99.18 0.0036
-
 2,6-dichlorophenolindophenol
-
 Phanerodontia chrysosporium
1.1.99.18 0.0052
-
 ferricyanide
-
 Phanerodontia chrysosporium
1.1.99.18 0.012
-
 di-t-butylbenzoqinone
-
 Phanerodontia chrysosporium
1.1.99.18 0.013
-
 cytochrome c
-
 Phanerodontia chrysosporium
1.1.99.18 0.034
-
 Fe3+
-
 Phanerodontia chrysosporium
1.1.99.18 0.12
-
 oxygen
-
 Phanerodontia chrysosporium
1.1.99.18 0.15
-
 Mn(III)-malonate
-
 Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 89170
-
 laser-induced desorption mass spectrometry Phanerodontia chrysosporium
1.1.99.18 90000
-
 1 * 90000, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.18 cellobiose + O2 Phanerodontia chrysosporium CBO binds strongly to cellulose and the radical reducing activity may decrease repolimerization and precipitation of lignin-like polymers on the cellulose surface, thereby facilitating cellulose degradation cellobiono-1,5-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Coniophora puteana
-
 produces cellobiose oxidase
-
1.1.99.18 Phanerodontia chrysosporium
-
 earlier name: Sporotrichum pulverulentum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18
-
 Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + Fe3+
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced Fe3+
-
 r
1.1.99.18 cellobiose + ferricyanide
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + ferrocyanide
-
 r
1.1.99.18 cellobiose + Mn(III)-malonate
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced Mn(III)-malonate
-
 r
1.1.99.18 cellobiose + O2
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + H2O2 enzyme produces H2O2 in a slow process ?
1.1.99.18 cellobiose + O2 CBO binds strongly to cellulose and the radical reducing activity may decrease repolimerization and precipitation of lignin-like polymers on the cellulose surface, thereby facilitating cellulose degradation Phanerodontia chrysosporium cellobiono-1,5-lactone + H2O2
-
 ?
1.1.99.18 lactose + 3,5-di-tert-butyl-1,2-benzoquinone
-
 Phanerodontia chrysosporium 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
1.1.99.18 additional information electron acceptors: great number of quinones and radicals, cytochrome c, Mn(III), ferricyanide, oxygen, reduction of Fe(III) and cytochrome c more rapid than of oxygen, enzyme reduces also compounds of ligninase and manganese peroxidase in absence of their substrates Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 monomer 1 * 90000, SDS-PAGE Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 FAD
-
 Phanerodontia chrysosporium
1.1.99.18 flavin flavohemoprotein Phanerodontia chrysosporium
1.1.99.18 heme b cleavage of heme containing fragment by papain or V8 proteinase can convert CBO to cellobiose:quinone dehydrogenase, cytochrome b-type heme group used for storage and delivery of electrons from a two-electron donor, e.g. cellobiose, via FADH2 to one-electron acceptors, e.g. radicals, cytochrome c, ferricyanide and oxygen Phanerodontia chrysosporium