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Literature summary extracted from
- Cellobiose oxidase from Sporotrichum pulverulentum (1982), Methods Enzymol., 89, 129-135.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | 2,2-bipyridine | - |
Phanerodontia chrysosporium |  |
| 1.1.99.18 | Sodium azide | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | Sodium cyanide | - |
Phanerodontia chrysosporium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 93000 | - |
sedimentation equilibrium centrifugation | Phanerodontia chrysosporium |
| 1.1.99.18 | 102000 | - |
1 * 102000, SDS-PAGE | Phanerodontia chrysosporium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + O2 | Phanerodontia chrysosporium | role in cellulose degradation | cellobiono-1,5-lactone + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Phanerodontia chrysosporium | - |
white-rot fungus | - |
| 1.1.99.18 | Phanerodontia chrysosporium | - |
strain ATCC 32629 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | glycoprotein | binds to immobilized concanavalin A | Phanerodontia chrysosporium |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | - |
Phanerodontia chrysosporium |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | culture filtrate | - |
Phanerodontia chrysosporium | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 0.417 | - |
- |
Phanerodontia chrysosporium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.99.18 | -20°C, 50 mM sodium acetate buffer, pH 5, 1 week, less than 10% loss of activity | Phanerodontia chrysosporium |
| 1.1.99.18 | 0°C, 50 mM sodium acetate buffer, pH 5, 1 week, less than 10% loss of activity | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | 4-beta-glucosylmannose + O2 | slowly oxidized | Phanerodontia chrysosporium | ? | - |
? | |
| 1.1.99.18 | cellobiose + O2 | enzyme consumes 1 mol molecular oxygen per mol of substrate oxidized | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | reaction product is cellobionic acid, no H2O2 detected, but superoxide anion | ? | |
| 1.1.99.18 | cellobiose + O2 | readily oxidized, cellobiose and cellohexaose with almost same activity | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | reaction product is cellobionic acid, no H2O2 detected, but superoxide anion | ? | |
| 1.1.99.18 | cellobiose + O2 | role in cellulose degradation | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
| 1.1.99.18 | cellodextrin + O2 | decreasing rapidity in the series from cellobiose to cellotetraose, but then with increasing size they are more rapidly oxidized | Phanerodontia chrysosporium | aldonic acid + H2O2 | - |
? | |
| 1.1.99.18 | galactose + O2 | slowly oxidized | Phanerodontia chrysosporium | ? | - |
? | |
| 1.1.99.18 | lactose + O2 | readily oxidized | Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2 | product is lactobionic acid | ? | |
| 1.1.99.18 | mannose + O2 | slowly oxidized | Phanerodontia chrysosporium | ? | - |
? | |
| 1.1.99.18 | additional information | not as substrates: maltose, sophorose, glucose, xylose, cellulose | Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | monomer | 1 * 102000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 30 | - |
assay at | Phanerodontia chrysosporium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 5 | - |
- |
Phanerodontia chrysosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | flavin | flavohemoprotein, one flavin per enzyme molecule | Phanerodontia chrysosporium | |
| 1.1.99.18 | heme b | flavohemoprotein | Phanerodontia chrysosporium | |
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Release 2023.1 (January 2023)
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