Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Banthorpe, D.V.; Cardemil, E.; Del Carmen Contraras, M.
    Purification and properties of alcohole oxidase from Tanacetum vulgare (1976), Phytochemistry, 15, 391-394.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.3.20 prolonged dialysis against EDTA, stable to Tanacetum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.20 additional information no inhibitor: cyanide; no inhibitors: iodoacetate, MnSO4, EDTA, p-hydroxymercuribenzoate, 2,2'-dipyridyl, 1,10-phenanthroline, 8-hydroxyquinoline, fluoride, azide Tanacetum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.20 0.19
-
 Hex-trans-2-ene-1-ol
-
 Tanacetum vulgare
1.1.3.20 0.49
-
 n-Octanol value above Tanacetum vulgare
1.1.3.20 1.56
-
 geraniol
-
 Tanacetum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.20 75000
-
 1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare
1.1.3.20 94000
-
 1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare
1.1.3.20 180000
-
 gel filtration Tanacetum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.20 long-chain alcohol + O2 Tanacetum vulgare substrates are primary alcohols with a chain-length of C6-C10 ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.3.20 Acetone stable to Tanacetum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.20 Tanacetum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.20 homogeneity Tanacetum vulgare

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.20 a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2 Tanacetum vulgare
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.20 leaf
-
 Tanacetum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.20 2.6
-
-
 Tanacetum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.20 geraniol + O2 i.e. 3,7-dimethylocta-trans-2,6-dien-1-ol, better substrate than cis-isomer, the 2-ene-group enhances activity Tanacetum vulgare 3,7-dimethylocta-trans-2,6-dien-1-al + H2O2
-
 ?
1.1.3.20 hex-trans-2-ene-1-ol + O2 preferred substrate, the 2-ene-group enhances activity Tanacetum vulgare hex-trans-2-ene-1-al + H2O2
-
 ?
1.1.3.20 long-chain alcohol + O2 substrates are primary alcohols with a chain-length of C6-C10 Tanacetum vulgare ?
-
 ?
1.1.3.20 additional information substrates are primary alcohols with a chain-length of C6-C10, optimal chain-length: C8-C10 Tanacetum vulgare ?
-
 ?
1.1.3.20 additional information no substrates for Tanacetum enzyme: secondary, tertiary alcohols and Tris Tanacetum vulgare ?
-
 ?
1.1.3.20 n-octanol + O2
-
 Tanacetum vulgare n-octanal + H2O2
-
 ?
1.1.3.20 terpenol + O2 poor substrate Tanacetum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.20 dimer 1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.20 18 22
-
 Tanacetum vulgare

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.20
-
 45 about 60% of maximal activity at 0°C and about 30% of maximal activity at 45°C Tanacetum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.20 5.5 9 plateau Tanacetum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.20 4.8 10 about half-maximal activity at pH 4.8 and 10.0 Tanacetum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.20 additional information NAD(P)+ Tanacetum vulgare
1.1.3.20 additional information FAD, FMN Tanacetum vulgare