EC Number | General Stability | Organism |
---|---|---|
1.1.3.19 | after solubilization the enzyme gradually loses activity when kept at 5°C, full reactivation by freezing and thawing | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.19 | 2,4-Dinitrophenylhydrazine | - |
Pseudomonas putida | |
1.1.3.19 | 2-mercaptoethanol | - |
Pseudomonas putida | |
1.1.3.19 | 8-hydroxyquinoline | - |
Pseudomonas putida | |
1.1.3.19 | Ag+ | - |
Pseudomonas putida | |
1.1.3.19 | Atebrin | - |
Pseudomonas putida | |
1.1.3.19 | Co2+ | - |
Pseudomonas putida | |
1.1.3.19 | Cu2+ | - |
Pseudomonas putida | |
1.1.3.19 | cysteine | - |
Pseudomonas putida | |
1.1.3.19 | diethyldithiocarbamate | - |
Pseudomonas putida | |
1.1.3.19 | dithiothreitol | - |
Pseudomonas putida | |
1.1.3.19 | DL-3,4-dihydroxymandelic acid | - |
Pseudomonas putida | |
1.1.3.19 | EDTA | - |
Pseudomonas putida | |
1.1.3.19 | Fe2+ | - |
Pseudomonas putida | |
1.1.3.19 | Fe3+ | - |
Pseudomonas putida | |
1.1.3.19 | guanidine hydrochloride | - |
Pseudomonas putida | |
1.1.3.19 | Hg2+ | - |
Pseudomonas putida | |
1.1.3.19 | hydroxylamine | - |
Pseudomonas putida | |
1.1.3.19 | potassium cyanide | - |
Pseudomonas putida | |
1.1.3.19 | reduced glutathione | - |
Pseudomonas putida | |
1.1.3.19 | SDS | - |
Pseudomonas putida | |
1.1.3.19 | Zn2+ | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.19 | 0.44 | - |
DL-4-hydroxymandelate | - |
Pseudomonas putida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.3.19 | membrane | bound | Pseudomonas putida | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.19 | Mn2+ | required | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.19 | 155000 | - |
gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.19 | (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 | Pseudomonas putida | enzyme of degradation pathway from mandelic acid to benzoic acid | 4-hydroxybenzaldehyde + CO2 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.19 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.19 | partial | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.19 | additional information | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.3.19 | -20°C, 2 months, solubilized enzyme | Pseudomonas putida |
1.1.3.19 | -20°C, particulate fraction suspended in 0.025 M sodium phosphate buffer, pH 7.0, more than 6 months | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.19 | (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 | absolute requirement for molecular oxygen, specific for L-isomer of 4-hydroxymandelic acid | Pseudomonas putida | 4-hydroxybenzaldehyde + CO2 + H2O2 | - |
? | |
1.1.3.19 | (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 | enzyme of degradation pathway from mandelic acid to benzoic acid | Pseudomonas putida | 4-hydroxybenzaldehyde + CO2 + H2O2 | - |
? | |
1.1.3.19 | DL-4-hydroxymandelate + ? | - |
Pseudomonas putida | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.3.19 | L-4-hydroxymandelate oxidase (decarboxylating) | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.19 | 55 | - |
- |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.19 | 6.6 | - |
- |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.19 | FAD | required | Pseudomonas putida |