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Literature summary extracted from

  • Koolman, J.; Karlson, P.
    Ecdysone Oxidase, an enzyme from the blowfly Calliphora erythrocephala (Meigen) (1975), Hoppe-Seyler's Z. Physiol. Chem., 356, 1131-1138.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.16 0.007
-
 oxidized 2,6-dichloroindophenol
-
 Calliphora vicina
1.1.3.16 0.042
-
 ecdysone
-
 Calliphora vicina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.16 240000
-
 gel filtration Calliphora vicina

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.16 Calliphora vicina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.16
-
 Calliphora vicina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.16 prepupa
-
 Calliphora vicina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.16 additional information
-
-
 Calliphora vicina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.16 ecdysone + O2 artificial electron acceptors in vitro in ascending order: ferricyanide, methylene blue, 2,6-dichloroindophenol, 5-methazine methyl sulfate Calliphora vicina 3-dehydroecdysone + H2O2
-
 ?
1.1.3.16 ecdysone + O2 2,6-dichloroindophenol can act as acceptor Calliphora vicina 3-dehydroecdysone + H2O2
-
 ?
1.1.3.16 ecdysone + O2 FAD, NAD+, NADP+, and FMN can serve as hydrogen acceptors Calliphora vicina 3-dehydroecdysone + H2O2
-
 ?
1.1.3.16 ecdysone + oxidized 2,6-dichloroindophenol 163% activity compared to O2 Calliphora vicina 3-dehydroecdysone + reduced 2,6-dichloroindophenol
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.16 45
-
-
 Calliphora vicina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.16 6.5
-
-
 Calliphora vicina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.16 5.3 8.6 pH 5.3: about 85% of activity maximum, pH 8.6: about 15% of activity maximum Calliphora vicina