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Literature summary extracted from

  • Artolozaga, M.J.; Kubatova, E.; Volc, J.; Kalisz, H.M.
    Pyranose 2-oxidase from Phanerochaete chrysosporium - further biochemical characterization (1997), Appl. Microbiol. Biotechnol., 47, 508-514.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.10 Ag+ completely inhibits at 10 mM Phanerodontia chrysosporium
1.1.3.10 ammonium sulfate slightly inhibits Phanerodontia chrysosporium
1.1.3.10 Cu2+ 50% inhibition at 10 mM Phanerodontia chrysosporium
1.1.3.10 Hg2+ completely inhibits at 10 mM Phanerodontia chrysosporium
1.1.3.10 Lead acetate 50% inhibition at 10 mM Phanerodontia chrysosporium
1.1.3.10 Mn2+ 50% inhibition at 10 mM Phanerodontia chrysosporium
1.1.3.10 Zn2+ completely inhibits at 10 mM Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.0832
-
 oxygen
-
 Phanerodontia chrysosporium
1.1.3.10 0.82
-
 D-glucose oxygen concentration 0.25 mM Phanerodontia chrysosporium
1.1.3.10 0.97
-
 beta-D-glucose oxygen concentration 1.07 mM Phanerodontia chrysosporium
1.1.3.10 1.09
-
 D-glucose oxygen concentration 1.07 mM Phanerodontia chrysosporium
1.1.3.10 1.43
-
 D-glucose at pH 8 Phanerodontia chrysosporium
1.1.3.10 1.53
-
 alpha-D-glucose oxygen concentration 1.07 mM Phanerodontia chrysosporium
1.1.3.10 25
-
 D-xylose oxygen concentration 1.07 mM Phanerodontia chrysosporium
1.1.3.10 55
-
 2-deoxy-D-glucose oxygen concentration 1.07 mM Phanerodontia chrysosporium
1.1.3.10 108
-
 L-sorbose oxygen concentration 1.07 mM Phanerodontia chrysosporium
1.1.3.10 240
-
 D-glucose at pH 4.5 Phanerodontia chrysosporium
1.1.3.10 257
-
 maltose oxygen concentration 1.07 mM Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 65000
-
 4 * 65000, SDS-PAGE Phanerodontia chrysosporium
1.1.3.10 250000
-
 PAGE under non-dissociating conditions Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 using liquid chromatography on phenyl Sepharose, Mono Q twice and phenyl Superose Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 31
-
-
 Phanerodontia chrysosporium

Storage Stability

EC Number Storage Stability Organism
1.1.3.10 -20°C, 50 mM Tris-HCl, pH 8, 30% glycerol, more than 1 year Phanerodontia chrysosporium
1.1.3.10 4°C, pH 4-11, retains more than 85% of the residual activity, 1 week Phanerodontia chrysosporium
1.1.3.10 repeated freezing at -20°C and thawing results in a rapid and irreversible loss of activity Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 1-beta-aurothioglucose + O2 91% relative activity to D-glucose Phanerodontia chrysosporium ?
-
 ?
1.1.3.10 2-deoxy-D-glucose + O2 25% relative activity to D-glucose Phanerodontia chrysosporium 2-deoxy-3-keto-D-glucose + H2O2
-
 ?
1.1.3.10 3-O-methyl-D-glucose + O2 8.6% relative activity to D-glucose Phanerodontia chrysosporium 2-keto-3-O-methyl-D-glucose + H2O2
-
 ?
1.1.3.10 5-thioglucose + O2 30% relative activity to D-glucose Phanerodontia chrysosporium 2-keto-5-thioglucose + H2O2
-
 ?
1.1.3.10 6-deoxy-D-glucose + O2 76% relative activity to D-glucose Phanerodontia chrysosporium 2-keto-6-deoxy-D-glucose + H2O2
-
 ?
1.1.3.10 alpha-D-glucose + O2
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 beta-D-glucose + O2
-
 Phanerodontia chrysosporium beta-2-dehydro-D-glucose + H2O2
-
 r
1.1.3.10 D-fructose + O2 6.5% relative activity to D-glucose Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 D-galactose + O2 4% relative activity to D-glucose Phanerodontia chrysosporium 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-gluconolactone + O2 44% relative activity to D-glucose Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 D-glucose + O2 D-glucose is the preferred substrate Phanerodontia chrysosporium D-glucosone + H2O2
-
 ?
1.1.3.10 D-glucose + O2 alpha-D-glucose has a 104% relative activity compared to D-glucose, beta-D-glucose has a 80% relative activity compared to D-glucose Phanerodontia chrysosporium D-glucosone + H2O2
-
 ?
1.1.3.10 D-xylose + O2 30% relative activity to D-glucose Phanerodontia chrysosporium D-xylosone + H2O2
-
 ?
1.1.3.10 L-sorbose + O2 68% relative activity to D-glucose Phanerodontia chrysosporium 5-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 maltose + O2 26% relative activity to D-glucose Phanerodontia chrysosporium ? + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 tetramer 4 * 65000, SDS-PAGE Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 55
-
 in 100 mM Tris, pH 8 Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 8 8.5
-
 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.10 5 9
-
 Phanerodontia chrysosporium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.10 4 4.5 50 mM acetate, the enzyme is relatively stable Phanerodontia chrysosporium
1.1.3.10 6
-
 2-(N-morpholino)ethane sulfonic acid, the enzyme is relatively stable Phanerodontia chrysosporium
1.1.3.10 6 7 bisTris, the enzyme is relatively stable Phanerodontia chrysosporium
1.1.3.10 8
-
 optimal stability, 50 mM Tris-HCl, triethanolamine and imidazole, more than 95% of the residual activity remains after 36 days Phanerodontia chrysosporium
1.1.3.10 8
-
 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid, the enzyme is relatively stable Phanerodontia chrysosporium