EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.10 | 1,10-phenanthroline | no significant effect | Phlebiopsis gigantea | |
1.1.3.10 | 2-mercaptoethanol | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea | |
1.1.3.10 | Ag+ | completely inhibits at 1 mM | Phlebiopsis gigantea | |
1.1.3.10 | alpha,alpha'-dipyridyl | no significant effect | Phlebiopsis gigantea | |
1.1.3.10 | ascorbic acid | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea | |
1.1.3.10 | Dithionite | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea | |
1.1.3.10 | dithiothreitol | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea | |
1.1.3.10 | Fe2+ | completely inhibits at 1 mM | Phlebiopsis gigantea | |
1.1.3.10 | N-bromosuccinimide | completely inhibitory | Phlebiopsis gigantea | |
1.1.3.10 | p-chloromercuribenzoate | completely inhibits at 1 mM | Phlebiopsis gigantea | |
1.1.3.10 | reduced glutathione | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.10 | 0.65 | - |
oxygen | in the presence of glucose | Phlebiopsis gigantea | |
1.1.3.10 | 1.1 | - |
D-glucose | - |
Phlebiopsis gigantea | |
1.1.3.10 | 29.4 | - |
D-xylose | - |
Phlebiopsis gigantea | |
1.1.3.10 | 50 | - |
L-sorbose | - |
Phlebiopsis gigantea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.10 | Ca2+ | slightly stimulates at 1 mM | Phlebiopsis gigantea | |
1.1.3.10 | Co2+ | slightly stimulates at 1 mM | Phlebiopsis gigantea | |
1.1.3.10 | Mg2+ | slightly stimulates at 1 mM | Phlebiopsis gigantea | |
1.1.3.10 | Zn2+ | slightly stimulates at 1 mM | Phlebiopsis gigantea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | 76000 | - |
4 * 76000, SDS-PAGE | Phlebiopsis gigantea |
1.1.3.10 | 322800 | - |
determined on the basis of its Stokes radius and sedimentation coefficient, dynamic light-scattering experiments, gradient-gel electrophoresis and cross-linking studies | Phlebiopsis gigantea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.10 | Phlebiopsis gigantea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.10 | using fractionated ammonium sulfate precipitation, chromatography on DEAE-Sephacel, Sephacryl S 300, S Sepharose and Q Sepharose | Phlebiopsis gigantea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | 10.4 | - |
- |
Phlebiopsis gigantea |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.3.10 | repeated freezing at -20°C and thawing results in a rapid and irreversible loss of activity | Phlebiopsis gigantea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.10 | alpha-D-melibiose + O2 | 1.9% relative activity to D-glucose | Phlebiopsis gigantea | ? + H2O2 | - |
? | |
1.1.3.10 | D-fucose + O2 | 2.9% relative activity to D-glucose | Phlebiopsis gigantea | ? | - |
? | |
1.1.3.10 | D-galactose + O2 | 7.7% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-D-galactose + H2O2 | - |
? | |
1.1.3.10 | D-gluconolactone + O2 | 1.5% relative activity to D-glucose | Phlebiopsis gigantea | ? + H2O2 | - |
? | |
1.1.3.10 | D-glucose + O2 | activation energy for the conversion of D-glucose is 34.6 kJ/mol | Phlebiopsis gigantea | D-glucosone + H2O2 | - |
? | |
1.1.3.10 | D-glucose + O2 | D-glucose is the preferred substrate | Phlebiopsis gigantea | D-glucosone + H2O2 | - |
? | |
1.1.3.10 | D-xylose + O2 | 50% relative activity to D-glucose | Phlebiopsis gigantea | D-xylosone + H2O2 | - |
? | |
1.1.3.10 | L-sorbose + O2 | 109% relative activity to D-glucose | Phlebiopsis gigantea | 5-dehydro-D-fructose + H2O2 | - |
? | |
1.1.3.10 | maltose + O2 | 8.2% relative activity to D-glucose | Phlebiopsis gigantea | ? + H2O2 | - |
? | |
1.1.3.10 | methyl alpha-D-glucoside + O2 | 1.9% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-alpha-D-methylglucoside + H2O2 | - |
? | |
1.1.3.10 | methyl beta-D-glucoside + O2 | 9.6% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-beta-D-methylglucoside + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.10 | tetramer | 4 * 76000, SDS-PAGE | Phlebiopsis gigantea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | 6 | 6.5 | - |
Phlebiopsis gigantea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.10 | FAD | covalently bound | Phlebiopsis gigantea | |
1.1.3.10 | FAD | 3.6 molecules FAD per molecule enzyme | Phlebiopsis gigantea |