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Literature summary extracted from

  • Biegert, T.; Altenschmidt, U.; Eckerskorn, C.; Fuchs, G.
    Purification and properties of benzyl alcohol dehydrogenase from a denitrifying Thauera sp (1995), Arch. Microbiol., 163, 418-423.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.90 5,5'-dithiobis-(2-nitrobenzoate)
-
 Thauera sp.
1.1.1.90 additional information no inhibition by O2, EDTA, 2,2'bipyridy, pyrazole Thauera sp.
1.1.1.90 p-chloromercuribenzoate
-
 Thauera sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.05
-
 NAD+
-
 Thauera sp.
1.1.1.90 0.3
-
 4-Hydroxybenzyl alcohol
-
 Thauera sp.
1.1.1.90 0.32
-
 benzyl alcohol
-
 Thauera sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.90 40000
-
 4 * 40000, SDS-PAGE Thauera sp.
1.1.1.90 160000
-
 gel filtration Thauera sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD+ Thauera sp.
-
 benzaldehyde + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Thauera sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.90 0.106
-
-
 Thauera sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 2-fluorobenzyl alcohol + NAD+
-
 Thauera sp. 2-fluorobenzaldehyde + NADH
-
 r
1.1.1.90 4-hydroxybenzyl alcohol + NAD+ 125% of benzyl alcohol activity Thauera sp. 4-hydroxybenzaldehyde + NADH
-
 r
1.1.1.90 benzyl alcohol + NAD+
-
 Thauera sp. benzaldehyde + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.90 tetramer 4 * 40000, SDS-PAGE Thauera sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.90 85
-
 benzyl alcohol
-
 Thauera sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.90 6
-
 reduction of benzaldehyde Thauera sp.
1.1.1.90 9.2
-
 benzyl alcohol + NAD+ Thauera sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.90 4.7 7.6 less than half-maximal activity above and below, reduction of benzaldehyde Thauera sp.
1.1.1.90 8.5 9.7 less than half-maximal activity above and below, oxidation of benzyl alcohol Thauera sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+
-
 Thauera sp.
1.1.1.90 NADH
-
 Thauera sp.