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Literature summary extracted from

  • Curtis, A.J.; Shirk, M.C.; Fall, R.
    Allylic or benzylic stabilization is essential for catalysis by bacterial benzyl alcohol dehydrogenases (1999), Biochem. Biophys. Res. Commun., 259, 220-223.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.90 expression in Escherichia coli Pseudomonas putida
1.1.1.90 expression in Escherichia coli Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.017
-
 3-methyl-2-buten-1-ol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.046
-
 benzyl alcohol
-
 Acinetobacter calcoaceticus
1.1.1.90 0.11
-
 3-methyl-2-buten-1-ol
-
 Pseudomonas putida
1.1.1.90 0.13
-
 benzyl alcohol
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD+ Pseudomonas putida
-
 benzaldehyde + NADH
-
 ?
1.1.1.90 benzyl alcohol + NAD+ Acinetobacter calcoaceticus
-
 benzaldehyde + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Acinetobacter calcoaceticus
-
-
-
1.1.1.90 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 (R)-perillyl alcohol + NAD+ 61% activity relative to benzyl alcohol Acinetobacter calcoaceticus (R)-perillaldehyde + NADH
-
 ?
1.1.1.90 (R)-perillyl alcohol + NAD+ 49% activity relative to benzyl alcohol Pseudomonas putida (R)-perillaldehyde + NADH
-
 ?
1.1.1.90 (S)-perillyl alcohol + NAD+ 85% activity relative to benzyl alcohol Acinetobacter calcoaceticus (S)-perillaldehyde + NADH
-
 ?
1.1.1.90 (S)-perillyl alcohol + NAD+ 68% activity relative to benzyl alcohol Pseudomonas putida (S)-perillaldehyde + NADH
-
 ?
1.1.1.90 3-methyl-2-buten-1-ol + NAD+ 210% activity relative to benzyl alcohol Acinetobacter calcoaceticus 3-methyl-2-buten-1-al + NADH
-
 ?
1.1.1.90 3-methyl-2-buten-1-ol + NAD+ 180% activity relative to benzyl alcohol Pseudomonas putida 3-methyl-2-buten-1-al + NADH
-
 r
1.1.1.90 4-aminobenzyl alcohol + NAD+ 270% activity relative to benzyl alcohol Acinetobacter calcoaceticus 4-aminobenzaldehyde + NADH
-
 r
1.1.1.90 4-aminobenzyl alcohol + NAD+ 280% activity relative to benzyl alcohol Pseudomonas putida 4-aminobenzaldehyde + NADH
-
 r
1.1.1.90 benzyl alcohol + NAD+
-
 Pseudomonas putida benzaldehyde + NADH
-
 ?
1.1.1.90 benzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus benzaldehyde + NADH
-
 ?
1.1.1.90 geraniol + NAD+ 68% activity relative to benzyl alcohol Pseudomonas putida geranial + NADH + H+
-
 ?
1.1.1.90 geraniol + NAD+ 63% activity relative to benzyl alcohol Acinetobacter calcoaceticus geranial + NADH + H+
-
 ?
1.1.1.90 nerol + NAD+ 25% activity relative to benzyl alcohol Acinetobacter calcoaceticus neral + NADH + H+
-
 ?
1.1.1.90 nerol + NAD+ 77% activity relative to benzyl alcohol Pseudomonas putida neral + NADH + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.90 18
-
 benzyl alcohol
-
 Pseudomonas putida
1.1.1.90 37
-
 3-methyl-2-buten-1-ol
-
 Acinetobacter calcoaceticus
1.1.1.90 41
-
 3-methyl-2-buten-1-ol
-
 Acinetobacter calcoaceticus
1.1.1.90 63
-
 benzyl alcohol
-
 Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+
-
 Pseudomonas putida
1.1.1.90 NAD+
-
 Acinetobacter calcoaceticus
1.1.1.90 NADH
-
 Pseudomonas putida
1.1.1.90 NADH
-
 Acinetobacter calcoaceticus