Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • MacKintosh, R.W.; Fewson, C.A.
    Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II from Acinetobacter calcoaceticus. Purification and preliminary characterization (1988), Biochem. J., 250, 743-751.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.2.1.28 dithiothreitol stabilizes Acinetobacter calcoaceticus
1.2.1.28 MgCl2 stabilizes Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.0152
-
 NAD+
-
 Acinetobacter calcoaceticus
1.1.1.90 0.0173
-
 benzyl alcohol
-
 Acinetobacter calcoaceticus
1.2.1.28 0.00057
-
 benzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.079
-
 NAD+
-
 Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.90 39700
-
 4 * 39700, SDS-PAGE, cross-linking experiments in vitro using dimethylsuberimidate Acinetobacter calcoaceticus
1.1.1.90 155000
-
 gel filtration Acinetobacter calcoaceticus
1.2.1.28 55000
-
 4 * 55000, dehydrogenase II, SDS-PAGE Acinetobacter calcoaceticus
1.2.1.28 225000
-
 dehydrogenase II, gel filtration Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD+ Acinetobacter calcoaceticus
-
 benzaldehyde + NADH
-
 r
1.2.1.28 benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
 benzoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Acinetobacter calcoaceticus
-
-
-
1.2.1.28 Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.90
-
 Acinetobacter calcoaceticus
1.2.1.28 dehydrogenase II Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.90 238
-
-
 Acinetobacter calcoaceticus
1.2.1.28 79
-
-
 Acinetobacter calcoaceticus

Storage Stability

EC Number Storage Stability Organism
1.2.1.28 -20°C, 100 mM potassium phosphate, pH 7.5, 2 mM dithiothreitol, 1 mM EDTA, 1 mM benzamidine, 1.2 mM phenylmethanesulfonylfluoride, 40% glycerol, 3 months, 10% loss of activity Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus benzaldehyde + NADH
-
 r
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.90 tetramer 4 * 39700, SDS-PAGE, cross-linking experiments in vitro using dimethylsuberimidate Acinetobacter calcoaceticus
1.2.1.28 tetramer 4 * 55000, dehydrogenase II, SDS-PAGE Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.90 8.9
-
 benzaldehyde + NADH Acinetobacter calcoaceticus
1.1.1.90 9.2
-
 benzyl alcohol + NAD+ Acinetobacter calcoaceticus
1.2.1.28 9.5
-
-
 Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+
-
 Acinetobacter calcoaceticus
1.1.1.90 NADH
-
 Acinetobacter calcoaceticus
1.2.1.28 NAD+
-
 Acinetobacter calcoaceticus