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Literature summary extracted from

  • Klasen, R.; Bringer-Meyer, S.; Sahm, H.
    Investigation of 5-ketogluconate formation by Gluconobacter oxydans (1992), DECHEMA Biotechnol. Conf., 5, 109-112.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.69 expression in Escherichia coli and Gluconobacter oxydans Gluconobacter oxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.69 Cu2+ 1 mM, 40% loss of activity Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.69 0.073
-
 NADP+
-
 Gluconobacter oxydans
1.1.1.69 20.6
-
 D-gluconate
-
 Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.69 cytoplasm
-
 Gluconobacter oxydans 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.69 Mn2+ 1 mM, increases the activity to 127% Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.69 75000
-
 gel filtration Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.69 Gluconobacter oxydans
-
 subsp. oxydans DSM3503
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.69
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.69 40
-
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.69 5-dehydro-D-gluconate + NADPH
-
 Gluconobacter oxydans D-gluconate + NADP+
-
 r
1.1.1.69 D-fructose + NADPH 10% of the activity with 5-dehydrydro-D-gluconate Gluconobacter oxydans 5-dehydro-D-fructose + NADP+
-
 ?
1.1.1.69 D-gluconate + NADP+
-
 Gluconobacter oxydans 5-dehydro-D-gluconate + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.69 dimer 2 * 75000, SDS-PAGE Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.69 5
-
 reduction of 5-dehydro-D-gluconate Gluconobacter oxydans
1.1.1.69 10
-
-
 Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.69 NADPH cofactor Gluconobacter oxydans