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Literature summary extracted from

  • Stuhlemmer, U.; Kreis, W.
    Cardenolide formation and activity of pregnane-modifying enzymes in cell suspension cultures, shoot cultures and leaves of Digitalis lanata (1996), Plant Physiol. Biochem., 34, 85-91.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 Mg2+ strong inhibition of oxidation of 3alpha-hydroxysteroids Digitalis lanata
1.1.1.277 Mg2+
-
 Digitalis lanata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol NADPH as cofactor Digitalis lanata 5829
-
1.1.1.277 soluble
-
 Digitalis lanata
-
-
1.3.1.22 microsome
-
 Digitalis lanata
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 5beta-pregnan-3,20-dione + NAD(P)H Digitalis lanata 3alpha-hydroxysteroid-5beta-oxidoreductase activity 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
 ?
1.1.1.277 3beta-hydroxy-5beta-pregnane-20-one + NADP+ Digitalis lanata biosynthesis of cardenolides 5beta-pregnane-3,20-dione + NADPH
-
 ?
1.3.1.22 progesterone + NADPH + H+ Digitalis lanata
-
 5alpha-pregnan-3-20-dione + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Digitalis lanata
-
 floxglove, NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity
-
1.1.1.277 Digitalis lanata
-
-
-
1.3.1.22 Digitalis lanata
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 cell suspension culture low activity Digitalis lanata
-
1.1.1.50 leaf low activity in young leaves Digitalis lanata
-
1.1.1.50 shoot culture Digitalis lanata
-
1.1.1.277 cell suspension culture
-
 Digitalis lanata
-
1.1.1.277 leaf
-
 Digitalis lanata
-
1.1.1.277 shoot light- and dark-grown Digitalis lanata
-
1.3.1.22 cell suspension culture
-
 Digitalis lanata
-
1.3.1.22 leaf not in young laeves, low activity Digitalis lanata
-
1.3.1.22 shoot culture, light grown Digitalis lanata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 1.56
-
 substrate 5beta-dihydroprogesterone, young leaves Digitalis lanata
1.1.1.50 869.8
-
 substrate 5beta-dihydroprogesterone, green shoot culture Digitalis lanata
1.1.1.277 0.0003
-
 cell suspension culture Digitalis lanata
1.1.1.277 0.00084
-
 leaf Digitalis lanata
1.3.1.22 0.00014
-
 extract from shoot culture Digitalis lanata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3alpha-hydroxy-5beta-pregnan-20-one + NADP+ inhibition by Mg2+ only in oxidative direction Digitalis lanata 5beta-pregnan-3,20-dione + NADPH
-
 r
1.1.1.50 5beta-pregnan-3,20-dione + NAD(P)H 3alpha-hydroxysteroid-5beta-oxidoreductase activity Digitalis lanata 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
 ?
1.1.1.50 5beta-pregnan-3,20-dione + NAD(P)H 3alpha-hydroxysteroid-5beta-oxidoreductase activity Digitalis lanata 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
 r
1.1.1.277 3beta-hydroxy-5beta-pregnane-20-one + NADP+ stereospecific Digitalis lanata 5beta-pregnane-3,20-dione + NADPH
-
 ?
1.1.1.277 3beta-hydroxy-5beta-pregnane-20-one + NADP+ biosynthesis of cardenolides Digitalis lanata 5beta-pregnane-3,20-dione + NADPH
-
 ?
1.3.1.22 progesterone + NADPH + H+
-
 Digitalis lanata 5alpha-pregnan-3-20-dione + NADP+
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.50 42
-
 assay at Digitalis lanata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NADPH
-
 Digitalis lanata
1.1.1.277 NADPH
-
 Digitalis lanata
1.3.1.22 NADPH
-
 Digitalis lanata