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Literature summary extracted from

  • Nanjo, H.; Terada, T.; Umemura, T.; Nishinaka, T.; Mizoguchi, T.; Nishihara, T.
    Characterization of bovine liver cytosolic 3alpha-hydroxysteroid dehydrogenase and its aldo-keto reductase activity (1992), Int. J. Biochem., 24, 815-820.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 17alpha-methyltestosterone competitive inhibition Bos taurus
1.1.1.50 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Bos taurus
1.1.1.50 ethacrynate
-
 Bos taurus
1.1.1.50 ethynylestradiol competitive inhibition Bos taurus
1.1.1.50 indomethacin
-
 Bos taurus
1.1.1.50 megestrol competitive inhibition Bos taurus
1.1.1.50 sulfhydryl oxidizing reagents
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 35000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 additional information Bos taurus broad substrate range ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 0.15
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3alpha-hydroxy-5beta-androstan-17-one + NADP+
-
 Bos taurus 5beta-androstan-3,17-dione + NADPH
-
 r
1.1.1.50 5alpha-androstan-3,17-dione + NADPH + H+
-
 Bos taurus 3alpha-hydroxy-5alpha-androstan-17-one + NADP+
-
 r
1.1.1.50 5beta-androstan-3,17-dione + NADPH
-
 Bos taurus 5beta-androstan-3alpha,17alpha-diol + NADP+
-
 r
1.1.1.50 5beta-androstan-3alpha-ol-17-one + NADPH
-
 Bos taurus 5beta-androstan-3alpha,17alpha-diol + NADP+
-
 r
1.1.1.50 9,10-phenanthrenequinone + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.50 androsterone + NAD(P)+
-
 Bos taurus 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
1.1.1.50 benzoylpyridine + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.50 camphorquinone + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.50 hydroxybutyrate + NADP+
-
 Bos taurus oxobutanoate + NADPH
-
 ?
1.1.1.50 menadione + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.50 additional information reduction of carbonyl compounds e.g. quinones, aliphatic-chain diketones, ketones Bos taurus ?
-
 ?
1.1.1.50 additional information can not catalyze the dehydrogenation of 3beta-hydroxysteroids, epiandrosterone and 17beta-hydroxysteroids, i.e. testosterone and 17beta-estradiol Bos taurus ?
-
 ?
1.1.1.50 additional information broad substrate range Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.50 monomer 1 * 35000, SDS-PAGE Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NADP+
-
 Bos taurus
1.1.1.50 NADPH
-
 Bos taurus