EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.50 | - |
Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.50 | stable in solution for a long period | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.50 | 0.011 | - |
NAD+ | - |
Pseudomonas putida | |
1.1.1.50 | 0.0136 | - |
deoxycholate | - |
Pseudomonas putida | |
1.1.1.50 | 0.015 | - |
cholate | - |
Pseudomonas putida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.50 | cytosol | - |
Pseudomonas putida | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 25000 | - |
non-denaturing gel electrophoresis and gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | additional information | Pseudomonas putida | belongs to the group of Y'bile acid binders, bile acid metabolizing enzymes | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.50 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.50 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 23.6 | - |
purified enzyme, substrate cholate with NAD+ | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.50 | -80°C, lyophilized or ammonium sulfate precipitated, more than 1 year | Pseudomonas putida |
1.1.1.50 | 4°C, diluted in Tris-HCl, pH 8.0, with ß-mercaptoethanol, 20 days | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine + NAD+ | - |
Pseudomonas putida | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl glycine + NADH | - |
? | |
1.1.1.50 | 6-ketolithocholate + NAD+ | - |
Pseudomonas putida | 5beta-cholan-3,6-dione-24-oic acid + NADH | - |
r | |
1.1.1.50 | androsterone + NAD(P)+ | - |
Pseudomonas putida | 5alpha-androstan-3,17-dione + NAD(P)H | - |
? | |
1.1.1.50 | chenodeoxycholic acid + NAD(P)+ | - |
Pseudomonas putida | 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
r | |
1.1.1.50 | cholic acid + NAD(P)+ | - |
Pseudomonas putida | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
1.1.1.50 | deoxycholic acid + NAD(P)+ | - |
Pseudomonas putida | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ | - |
? | |
1.1.1.50 | additional information | belongs to the group of Y'bile acid binders, bile acid metabolizing enzymes | Pseudomonas putida | ? | - |
? | |
1.1.1.50 | taurocholic acid + NAD(P)+ | - |
Pseudomonas putida | 2[[7alpha,12alpha-dihydroxy-3,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADPH | - |
? | |
1.1.1.50 | ursodeoxycholate + NAD+ | - |
Pseudomonas putida | 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.50 | monomer | 1 * 25000, SDS-PAGE | Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 45 | - |
heating for 10 min, rapid heat denaturation over 50°C | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 10 | 11 | oxidation of cholate | Pseudomonas putida |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 6 | 10 | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.50 | NAD+ | specific, enzyme does not use NADP+ | Pseudomonas putida |