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Literature summary extracted from

  • Adachi, O.; Toyama, H.; Matsushita, K.
    Purification and properties of 3alpha-hydroxysteroid dehydrogenase from Pseudomonas putida (1997), Biosci. Biotechnol. Biochem., 61, 131-137.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.50
-
Pseudomonas putida

General Stability

EC Number General Stability Organism
1.1.1.50 stable in solution for a long period Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.011
-
NAD+
-
Pseudomonas putida
1.1.1.50 0.0136
-
deoxycholate
-
Pseudomonas putida
1.1.1.50 0.015
-
cholate
-
Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol
-
Pseudomonas putida 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 25000
-
non-denaturing gel electrophoresis and gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 additional information Pseudomonas putida belongs to the group of Y'bile acid binders, bile acid metabolizing enzymes ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 23.6
-
purified enzyme, substrate cholate with NAD+ Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.1.50 -80°C, lyophilized or ammonium sulfate precipitated, more than 1 year Pseudomonas putida
1.1.1.50 4°C, diluted in Tris-HCl, pH 8.0, with ß-mercaptoethanol, 20 days Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine + NAD+
-
Pseudomonas putida 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl glycine + NADH
-
?
1.1.1.50 6-ketolithocholate + NAD+
-
Pseudomonas putida 5beta-cholan-3,6-dione-24-oic acid + NADH
-
r
1.1.1.50 androsterone + NAD(P)+
-
Pseudomonas putida 5alpha-androstan-3,17-dione + NAD(P)H
-
?
1.1.1.50 chenodeoxycholic acid + NAD(P)+
-
Pseudomonas putida 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
r
1.1.1.50 cholic acid + NAD(P)+
-
Pseudomonas putida 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
?
1.1.1.50 deoxycholic acid + NAD(P)+
-
Pseudomonas putida 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
?
1.1.1.50 additional information belongs to the group of Y'bile acid binders, bile acid metabolizing enzymes Pseudomonas putida ?
-
?
1.1.1.50 taurocholic acid + NAD(P)+
-
Pseudomonas putida 2[[7alpha,12alpha-dihydroxy-3,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADPH
-
?
1.1.1.50 ursodeoxycholate + NAD+
-
Pseudomonas putida 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.50 monomer 1 * 25000, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.50 45
-
heating for 10 min, rapid heat denaturation over 50°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 10 11 oxidation of cholate Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.50 6 10
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NAD+ specific, enzyme does not use NADP+ Pseudomonas putida