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Literature summary extracted from

  • Iyer, R.B.; Binstock, J.M.; Schwartz, I.S.; Gordon, G.G.; Weinstein, B.I.; Southern, A.L.
    Purification and properties of human hepatic 3alpha-hydroxysteroid dehydrogenase (1992), J. Steroid Biochem. Mol. Biol., 43, 343-349.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.50 stable in phosphate 50 mM plus 10-20% glycerol at 4°C, while freezing and thawing results in a great loss of enzyme activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 dexamethasone
-
 Homo sapiens
1.1.1.50 dexamethasone 21-acetate
-
 Homo sapiens
1.1.1.50 dexamethasone 21-hemisuccinate
-
 Homo sapiens
1.1.1.50 testosterone at concentration 0.001 mM 75% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.018
-
 5beta-Dihydrocortisol
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 37000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 5alpha-dihydrocortisol + NADPH
-
 Homo sapiens 3alpha,5alpha-tetrahydrocortisol + NADP+
-
 ?
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 ?
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Homo sapiens (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
1.1.1.50 5beta-dihydrocortisol + NADPH
-
 Homo sapiens 3alpha,5beta-tetrahydrocortisol + NADP+
-
 ?
1.1.1.50 5beta-dihydrotestosterone + NAD(P)H
-
 Homo sapiens 5beta-androstan-3alpha,17beta-diol + NAD(P)+
-
 ?
1.1.1.50 additional information a saturated 4,5 double bound is essential for enzyme activity, 3-ketosteroids are not used as substrates Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.50 monomer 1 * 37000, SDS-PAGE Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.50 6.8 7.4
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NADPH preference for Homo sapiens