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Literature summary extracted from

  • Ikeda, M.; Hattori, H.; Ikeda, N.; Hayakawa, S.; Ohmori, S.
    Purification and characterization of the multiple forms of 3alpha-hydroxysteroid dehydrogenase in rat liver cytosol (1984), Hoppe-Seyler's Z. Physiol. Chem., 365, 377-391.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 additional information not inhibitory: phenobarbital, pyrazole Rattus norvegicus
1.1.1.50 p-chloromercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 additional information
-
 additional information values for various substrates and different isozymes Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 32500 34000 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50 multiple forms Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.50 -20°C, 25% v/v glycerol, at least 1 year or at 4°C, 1 week, stability depends on isozyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 androsterone + NAD(P)+ i.e. 3alpha-hydroxy-5alpha-androstan-17-one Rattus norvegicus 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
1.1.1.50 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Rattus norvegicus 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
1.1.1.50 glycolithocholic acid + NADP+ i.e. 3alpha-hydroxy-5beta-cholanoyl glycine Rattus norvegicus 3-oxo-glycocholanoic acid + NADPH
-
 ?
1.1.1.50 lithocholic acid + NADP+ i.e. 3alpha-hydroxy-5beta-cholan-24-oic acid Rattus norvegicus 3-oxo-5beta-cholan-24-oic acid + NADPH
-
 ?
1.1.1.50 additional information substrate specificity of various isozymes Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.50 additional information
-
 additional information overview, values for various substrates and 6 different isozymes Rattus norvegicus
1.1.1.50 0.444 2.6 androsterone
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 8.5
-
 oxidation with NAD+ Rattus norvegicus
1.1.1.50 10
-
 oxidation with NADP+ Rattus norvegicus