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Literature summary extracted from

  • Penning, T.M.; Sharp, R.B.; Krieger, N.R.
    Purification and properties of 3alpha-hydroxysteroid dehydrogenase from rat brain cytosol. Inhibition by nonsteroidal anti-inflammatory drugs and progestins (1985), J. Biol. Chem., 260, 15266-15272.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 1-Methylpyrrole acetic acid
-
 Rattus norvegicus
1.1.1.50 acetaminophen
-
 Rattus norvegicus
1.1.1.50 arylpropionic acid
-
 Rattus norvegicus
1.1.1.50 Fenamates
-
 Rattus norvegicus
1.1.1.50 indomethacin
-
 Rattus norvegicus
1.1.1.50 Medroxyprogesterone acetate
-
 Rattus norvegicus
1.1.1.50 Norethindrone
-
 Rattus norvegicus
1.1.1.50 progesterone
-
 Rattus norvegicus
1.1.1.50 Salicylates
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.007 0.0083 5alpha-dihydrotestosterone
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 31000
-
 1 * 31000, SDS-PAGE Rattus norvegicus
1.1.1.50 32500 34000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ Rattus norvegicus
-
 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 2.2
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.50 -80°C, 20 mM potassium phosphate buffer, pH 7.0, 0.1 M EDTA, 1 mM mercaptoethanol, 20% glycerol, 3 months, 10-15% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ i.e. 5alpha-androstan-17beta-ol-3-one Rattus norvegicus 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ i.e. 5alpha-androstan-17beta-ol-3-one Rattus norvegicus (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.50 monomer 1 * 31000, SDS-PAGE Rattus norvegicus