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Literature summary extracted from

  • Horiuchi, T.; Kurokawa, T.
    Purification and properties of N-acyl-D-mannosamine dehydrogenase from Flavobacterium sp. 141-8 (1988), J. Biochem., 104, 466-471.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.233 analysis quantitative determination of N-acetylneuraminic acid Flavobacterium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.233 cloned and expressed under control of a lac-promoter in Escherichia coli JM109, the Escherichia coli transformants JM109(pNAM307) and JM109(pNAM308) show up to 200-fold higher activity than Flavobacterium sp. Flavobacterium sp.

General Stability

EC Number General Stability Organism
1.1.1.233 inactivated by frequent freezing and thawing Flavobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.233 AMP slight inhibition Flavobacterium sp.
1.1.1.233 Cd2+ slight inhibition Flavobacterium sp.
1.1.1.233 Cu2+ slight inhibition Flavobacterium sp.
1.1.1.233 Hg2+ strong inhibitor Flavobacterium sp.
1.1.1.233 Mn2+ slight inhibition Flavobacterium sp.
1.1.1.233 additional information no inhibition with metal-chelating or SH-group-blocking reagents, e.g. EDTA, 2,2'-bipyridyl, 8-hydroxyquinoline, PCMB Flavobacterium sp.
1.1.1.233 Ni2+ slight inhibition Flavobacterium sp.
1.1.1.233 SDS strong inhibitor Flavobacterium sp.
1.1.1.233 Zn2+ slight inhibition Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.233 0.41
-
NAD+
-
Flavobacterium sp.
1.1.1.233 1
-
N-acetyl-D-mannosamine
-
Flavobacterium sp.
1.1.1.233 13.3
-
N-glycolyl-D-mannosamine
-
Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.233 27470
-
calculated from amino acid sequence Flavobacterium sp.
1.1.1.233 29000
-
SDS-PAGE Flavobacterium sp.
1.1.1.233 120000
-
gel filtration Flavobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.233 N-acetyl-D-mannosamine + NAD+ Flavobacterium sp. reaction in N-acetyl-D-mannosamine catabolism N-acetyl-D-mannosaminolactone + NADH + H+
-
?
1.1.1.233 N-acetyl-D-mannosamine + NAD+ Flavobacterium sp. 141-8 reaction in N-acetyl-D-mannosamine catabolism N-acetyl-D-mannosaminolactone + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.233 Flavobacterium sp.
-
141-8
-
1.1.1.233 Flavobacterium sp. 141-8
-
141-8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.233
-
Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.233 0.13
-
crude extract Flavobacterium sp.
1.1.1.233 340
-
purified enzyme Flavobacterium sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.233 -20°C, stable for at least several months Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.233 N-acetyl-D-mannosamine + NAD+
-
Flavobacterium sp. N-acetyl-D-mannosaminolactone + NADH + H+
-
ir
1.1.1.233 N-acetyl-D-mannosamine + NAD+ reaction in N-acetyl-D-mannosamine catabolism Flavobacterium sp. N-acetyl-D-mannosaminolactone + NADH + H+
-
?
1.1.1.233 N-acetyl-D-mannosamine + NAD+
-
Flavobacterium sp. 141-8 N-acetyl-D-mannosaminolactone + NADH + H+
-
ir
1.1.1.233 N-acetyl-D-mannosamine + NAD+ reaction in N-acetyl-D-mannosamine catabolism Flavobacterium sp. 141-8 N-acetyl-D-mannosaminolactone + NADH + H+
-
?
1.1.1.233 N-glycolyl-D-mannosamine + NAD+ oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation Flavobacterium sp. N-glycolyl-D-mannosaminolactone + NADH
-
?
1.1.1.233 N-glycolyl-D-mannosamine + NAD+ oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation Flavobacterium sp. 141-8 N-glycolyl-D-mannosaminolactone + NADH
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.233 tetramer 4 * 29000 Flavobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.233 25
-
inactivation at pH 5.0 after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h Flavobacterium sp.
1.1.1.233 42
-
and below, stable for at least 10 min Flavobacterium sp.
1.1.1.233 50
-
t1/2: 10 min Flavobacterium sp.
1.1.1.233 60
-
complete inactivation after 10 min Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.233 8 9.5
-
Flavobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.233 6 11.5
-
Flavobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.233 5
-
inactivation at 25°C after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h Flavobacterium sp.
1.1.1.233 7
-
and below, more than 70% loss of activity Flavobacterium sp.
1.1.1.233 8.5 9.5 more stable than at pH 8.2 at 45°C, 10 min Flavobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.233 additional information no cofactor: NADP+, ferricyanide, 2,6-dichlorophenolindophenol, phenazine methosulfate Flavobacterium sp.
1.1.1.233 NAD+ specific for Flavobacterium sp.