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Literature summary extracted from

  • Furuichi, A.; Akita, H.; Matsukura, H.; Oishi, T.; Horikoshi, K.
    Purification and properties of an asymmetric reduction of diethyl 2-methyl-3-oxosuccinate in Saccharomyces fermentati (1987), Agric. Biol. Chem., 51, 293-299.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.229 1,10-phenanthroline 30% of activity at 10 mM Torulaspora delbrueckii
1.1.1.229 cysteine complete inhibition at 10 mM Torulaspora delbrueckii
1.1.1.229 FMN slight inhibition at 0.2 mM Torulaspora delbrueckii
1.1.1.229 high salt concentration 1M NaCl or KCl Torulaspora delbrueckii
1.1.1.229 KCN complete inhibition at 10 mM Torulaspora delbrueckii
1.1.1.229 monoiodoacetate complete inhibition at 10 mM Torulaspora delbrueckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.229 1.25
-
 Diethyl 2-methyl-3-oxosuccinate
-
 Torulaspora delbrueckii
1.1.1.229 111
-
 Diethyl 2-methyl-3-hydroxysuccinate
-
 Torulaspora delbrueckii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.229 Ag+ strong inhibitor Torulaspora delbrueckii
1.1.1.229 Cu2+ strong inhibitor Torulaspora delbrueckii
1.1.1.229 Fe3+ strong inhibitor Torulaspora delbrueckii
1.1.1.229 K+ strongly inhibitory at 1 M Torulaspora delbrueckii
1.1.1.229 Na+ strongly inhibitory at 1 M Torulaspora delbrueckii
1.1.1.229 Zn2+ slight inhibition Torulaspora delbrueckii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.229 61000
-
 gel filtration Torulaspora delbrueckii
1.1.1.229 63000
-
 SDS-PAGE Torulaspora delbrueckii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.229 diethyl 2-methyl-3-oxosuccinate + NADPH Torulaspora delbrueckii asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity diethyl 2-methyl-3-hydroxysuccinate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.229 Torulaspora delbrueckii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.229
-
 Torulaspora delbrueckii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.229 0.004
-
 crude extract Torulaspora delbrueckii
1.1.1.229 0.649
-
 purified enzyme Torulaspora delbrueckii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.229 diethyl 2-methyl-3-oxosuccinate + NADPH the reduction is strongly preferred, the catalyzed reaction is an asymmetric reduction, best substrate Torulaspora delbrueckii diethyl 2-methyl-3-hydroxysuccinate + NADP+ alpha-methyl-beta-hydroxy ester, the enzyme catalyzes an asymmetric reduction, the product is a mixture of the (2R,3R)-syn- and (2S,3R)-anti-beta-hydroxyester r
1.1.1.229 diethyl 2-methyl-3-oxosuccinate + NADPH asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity Torulaspora delbrueckii diethyl 2-methyl-3-hydroxysuccinate + NADP+
-
 r
1.1.1.229 dimethyl 2-methyl-3-oxosuccinate + NADPH poor substrate Torulaspora delbrueckii dimethyl 2-methyl-3-hydroxysuccinate + NADP+
-
 ?
1.1.1.229 additional information no aldehydes or several synthetic substrates are reduced, such as: acetaldehyde, formaldehyde, isobutanal, n-pentanal, n-hexanal, n-heptanal, 4-hydroxybenzaldehyde, 4-dimethylaminobenzaldehyde, benzaldehyde, furfural, 2-methyl-3-oxobutanoic acid benzyl ester, 2-chloro-3-oxobutanoic acid ethyl ester, 3-phenyl-2-oxobutanoic acid methyl ester, 2-methyl-3-oxobutanoic acid ethyl ester, 3-(2'-furyl)-2-methyl-3-oxopropionic acid methyl ester, none of the following alcohols or other substrates is oxidized: methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, benzyl alcohol, glycerin, shikimic acid, 2-deoxy-D-glucose, glucose 6-phosphate, in the presence of NAD+ or NADP+ Torulaspora delbrueckii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.229 monomer
-
 Torulaspora delbrueckii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.229 50
-
-
 Torulaspora delbrueckii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.229 30 60 more than 70% of maximal activity at both temperatures Torulaspora delbrueckii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.229 40
-
 very unstable at temperatures above 40°C for 10 min Torulaspora delbrueckii
1.1.1.229 60
-
 complete loss of activity Torulaspora delbrueckii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.229 6
-
 sharp peak Torulaspora delbrueckii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.229 4 8 less than 10% of activity at both pH Torulaspora delbrueckii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.229 6 9 stable for 30 min Torulaspora delbrueckii