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Literature summary extracted from

  • Watanabe, K.; Yoshida, R.; Shimizu, T.; Hayaishi, O.
    Enzymatic formation of prostaglandin F2 alpha from prostaglandin H2 and D2. Purification and properties of prostaglandin F synthetase from bovine lung (1985), J. Biol. Chem., 260, 7035-7041.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.188 0.0007
-
 9,10-phenanthrenequinone native lung prostaglandin F synthase Bos taurus
1.1.1.188 0.01
-
 prostaglandin H2 native lung prostaglandin F synthase Bos taurus
1.1.1.188 0.013
-
 4-Nitroacetophenone lung enzyme Bos taurus
1.1.1.188 0.018
-
 Hydrindantin lung enzyme Bos taurus
1.1.1.188 0.08
-
 Phenylglyoxal lung enzyme Bos taurus
1.1.1.188 0.1
-
 Prostaglandin D2 lung enzyme Bos taurus
1.1.1.188 0.125
-
 4-nitrobenzaldehyde lung enzyme Bos taurus
1.1.1.188 0.25
-
 Prostaglandin A2 lung enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.188 30500
-
 SDS-PAGE, sedimentation equilibrium and gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.188
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 lung two different active sites Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.188 0.15
-
 prostaglandin F2alpha as product Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
1.1.1.188 9,10-phenanthrenequinone + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 hydrindantin + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 phenylglyoxal + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 prostaglandin A2 + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.188 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.188 6 7
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADPH
-
 Bos taurus