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Literature summary extracted from

  • Watanabe, K.; Fujii, Y.; Ohkubo, H.; Kuramitsu, S.; Kagamiyama, H.; Nakanishi, S.; Hayaishi, O.
    Expression of bovine lung prostaglandin F synthase in Escherichia coli (1991), Biochem. Biophys. Res. Commun., 181, 272-278.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 expression in Escherichia coli Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.188 0.0001
-
 9,10-phenanthrenequinone expressed lung enzyme Bos taurus
1.1.1.188 0.008
-
 prostaglandin H2 expressed lung enzyme Bos taurus
1.1.1.188 0.1
-
 Prostaglandin D2 expressed lung enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.188 36000
-
 gel filtration Bos taurus
1.1.1.188 36000
-
 SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Bos taurus
-
 two isozymes, lung-type and liver-type
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.188 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 lung two different active sites Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.188 0.115
-
 4-nitrobenzaldehyde reductase activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
1.1.1.188 9,10-phenanthrenequinone + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 additional information two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds Bos taurus ?
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.188 monomer 1 * 36000, SDS-PAGE Bos taurus
1.1.1.188 monomer 1 * 36000, gel filtration Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADPH
-
 Bos taurus