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Literature summary extracted from

  • Kuchinke, W.; Barski, O.; Watanabe, K.; Hayaishi, O.
    A lung type prostaglandin F synthase is expressed in bovine liver: cDNA sequence and expression in E. coli (1992), Biochem. Biophys. Res. Commun., 183, 1238-1246.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 expression in Escherichia coli Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.188 0.00013
-
 9,10-phenanthrenequinone lung enzyme Bos taurus
1.1.1.188 0.0008
-
 4-nitrobenzaldehyde liver enzyme Bos taurus
1.1.1.188 0.001
-
 9,10-phenanthrenequinone liver enzyme Bos taurus
1.1.1.188 0.0083
-
 4-nitrobenzaldehyde lung enzyme Bos taurus
1.1.1.188 0.013
-
 menadione lung enzyme Bos taurus
1.1.1.188 0.1
-
 menadione liver enzyme Bos taurus
1.1.1.188 0.1
-
 Prostaglandin D2 lung enzyme Bos taurus
1.1.1.188 0.2
-
 Prostaglandin D2 liver enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.188 36000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Bos taurus
-
 two isozymes, lung-type and liver-type
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.188
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 liver
-
 Bos taurus
-
1.1.1.188 lung
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.188 0.034
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
1.1.1.188 4-nitrobenzaldehyde + NADPH
-
 Bos taurus 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.188 9,10-phenanthrenequinone + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 menadione + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.188 monomer 1 * 36000, gel filtration Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADPH
-
 Bos taurus