EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.188 | expression in COS-7 cells | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.188 | H170C | fully active | Bos taurus |
1.1.1.188 | K225S | 2fold as active as wild-type | Bos taurus |
1.1.1.188 | N306Y | half as active as wild-type | Bos taurus |
1.1.1.188 | R223L | 5fold as active as wild-type | Bos taurus |
1.1.1.188 | R27E | fully active | Bos taurus |
1.1.1.188 | R91Q | fully active | Bos taurus |
1.1.1.188 | S301R | 2.5fold as active as wild-type | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.188 | 0.0007 | - |
9,10-phenanthrenequinone | native lung prostaglandin F synthase | Bos taurus | |
1.1.1.188 | 0.0011 | - |
9,10-phenanthrenequinone | expressed liver prostaglandin F synthase | Bos taurus | |
1.1.1.188 | 0.002 | - |
9,10-phenanthrenequinone | native liver prostaglandin F synthase | Bos taurus | |
1.1.1.188 | 0.01 | - |
prostaglandin H2 | native lung prostaglandin F synthase | Bos taurus | |
1.1.1.188 | 0.01 | - |
Prostaglandin D2 | native liver prostaglandin F synthase | Bos taurus | |
1.1.1.188 | 0.015 | - |
Prostaglandin D2 | expressed liver prostaglandin F synthase | Bos taurus | |
1.1.1.188 | 0.025 | - |
prostaglandin H2 | native and expressed liver prostaglandin F synthase | Bos taurus | |
1.1.1.188 | 0.12 | - |
Prostaglandin D2 | native lung prostaglandin F synthase | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.188 | 36740 | - |
amino acid analysis, SDS-PAGE | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.188 | Bos taurus | - |
two isozymes, lung-type and liver-type | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.188 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.188 | liver | - |
Bos taurus | - |
1.1.1.188 | lung | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.188 | (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH | - |
Bos taurus | (5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+ | - |
? | |
1.1.1.188 | 9,10-phenanthrenequinone + NADPH | - |
Bos taurus | ? | - |
? | |
1.1.1.188 | prostaglandin H2 + NADPH + H+ | - |
Bos taurus | (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.188 | NADPH | - |
Bos taurus |