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Literature summary extracted from

  • Kubata, B.K.; Duszenko, M.; Kabututu, Z.; Rawer, M.; Szallies, A.; Fujimori, K.; Inui, T.; Nozaki, T.; Yamashita, K.; Horii, T.; Urade, Y.; Hayaishi, O.
    Identification of a novel prostaglandin F2.alpha. synthase in Trypanosoma brucei (2000), J. Exp. Med., 192, 1327-1337.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 expression in Escherichia coli Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.188 0.00042
-
 9,10-phenanthrenequinone
-
 Trypanosoma brucei
1.1.1.188 0.0008
-
 4-nitrobenzaldehyde
-
 Trypanosoma brucei
1.1.1.188 0.0013
-
 prostaglandin H2
-
 Trypanosoma brucei
1.1.1.188 0.0057
-
 testosterone
-
 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.188 cytosol
-
 Trypanosoma brucei 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.188 33000
-
 SDS-PAGE Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.188
-
 Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.188 0.86
-
-
 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 4-nitrobenzaldehyde + NADPH
-
 Trypanosoma brucei 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.188 9,10-phenanthrenequinone + NADPH
-
 Trypanosoma brucei ?
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Trypanosoma brucei (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
1.1.1.188 testosterone + NADPH + H+
-
 Trypanosoma brucei ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.188 37
-
 assay at Trypanosoma brucei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.188 25 40
-
 Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.188 7
-
 assay at Trypanosoma brucei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.188 6 9
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADPH
-
 Trypanosoma brucei