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Literature summary extracted from

  • Wolff, J.B.; Kaplan, N.O.
    D-Mannitol-1-phosphate dehydrogenase from E. coli (1955), Methods Enzymol., 1, 346-348.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.17 intracellular
-
 Escherichia coli 5622
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Escherichia coli highly specific for both substrates D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ Escherichia coli favours reverse reaction D-fructose 6-phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 precipitation techniques Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.17 cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 additional information
-
 1 unit defined as change of 0.01 in optical density at 340 nm during 30-90 second interval Escherichia coli
1.1.1.17 additional information
-
 860 U/mg protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ highly specific for both substrates Escherichia coli D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ favours reverse reaction Escherichia coli D-fructose 6-phosphate + NADH + H+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.17 55
-
 45% loss of activity in 10 min Escherichia coli
1.1.1.17 60
-
 complete inactivation in 10 min Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.17 6 9 no sharp optimum Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+ strictly specific Escherichia coli
1.1.1.17 NADH strictly specific Escherichia coli