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Literature summary extracted from

  • Brown, A.T.; Bowles, R.D.
    Polyol metabolism by a caries-conductive Streptococcus: Purification and properties of a nicotinamide adenine dinucleotide-dependent mannitol-1-phosphate dehydrogenase (1977), Infect. Immun., 16, 163-173.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 adenosine 48% inhibition of fructose 6-phosphate reduction Streptococcus mutans
1.1.1.17 ADP 20% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
1.1.1.17 ATP 53% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
1.1.1.17 cytidine 17% inhibition of fructose 6-phosphate reduction Streptococcus mutans
1.1.1.17 D-fructose 1,6-diphosphate 12% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
1.1.1.17 D-fructose 6-phosphate 56% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
1.1.1.17 GTP 11% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
1.1.1.17 guanosine 10% inhibition of fructose 6-phosphate reduction Streptococcus mutans
1.1.1.17 phosphoenolpyruvate 14% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
1.1.1.17 thymidine 8% inhibition of fructose 6-phosphate reduction Streptococcus mutans
1.1.1.17 uridine 16% inhibition of fructose 6-phosphate reduction Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.17 0.016
-
 NADH cosubstrate: D-fructose 6-phosphate Streptococcus mutans
1.1.1.17 0.06
-
 NAD+ cosubstrate: D-mannitol 1-phosphate Streptococcus mutans
1.1.1.17 0.15
-
 D-mannitol 1-phosphate cosubstrate: NAD+ Streptococcus mutans
1.1.1.17 1.66
-
 D-fructose 6-phosphate cosubstrate: NADH Streptococcus mutans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.17 intracellular
-
 Streptococcus mutans 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 45000
-
 gel filtration Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Streptococcus mutans highly specific for both substrates D-fructose 6-phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Streptococcus mutans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 to homogeneity, chromatography steps, affinity chromatography Streptococcus mutans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.17 cell culture
-
 Streptococcus mutans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 0.43
-
-
 Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
1.1.1.17 -80°C, 0.05 M Tris-HCl, pH 7.5, presence of 2-mercaptoethanol, long term storage without loss of activity Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ highly specific for both substrates Streptococcus mutans D-fructose 6-phosphate + NADH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 6.5
-
 reduction of D-fructose 6-phosphate Streptococcus mutans
1.1.1.17 8.5 9 oxidation of D-mannitol 1-phosphate Streptococcus mutans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+ strictly specific Streptococcus mutans
1.1.1.17 NADH
-
 Streptococcus mutans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.17 2
-
 ADP competitive inhibition with respect to NADH, noncompetitive inhibition with respect to fructose 6-phosphate Streptococcus mutans
1.1.1.17 2.2
-
 ATP strong inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
1.1.1.17 3.33
-
 D-fructose 6-phosphate able to inhibit the forward reaction to 100%, competitive inhibition with respect to mannitol 1-phosphate, noncompetitive inhibition with respect to NAD+ Streptococcus mutans