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Literature summary extracted from

  • Nash, W.E.; Mercer, R.W.; Blanco, G.; Strickler, R.C.; Mason, J.I.; Thomas, J.L.
    Over-expression of human type I (placental) 3beta-hydroxy-5-ene-steroid dehydrogenase/isomerase in insect cells infected with recombinant baculovirus (1994), J. Steroid Biochem. Mol. Biol., 50, 235-240.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 expression in Spodoptera fungiperda insect cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0144
-
 5-androstene-3,17-dione placental microsomes Homo sapiens
1.1.1.145 0.0147
-
 5-androstene-3,17-dione Sf9 cell homogenate Homo sapiens
1.1.1.145 0.0167
-
 5alpha-Androstan-3beta-ol-17-one placental microsomes Homo sapiens
1.1.1.145 0.0179
-
 5alpha-Androstan-3beta-ol-17-one Sf9 cell homogenate Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.145 microsome
-
 Homo sapiens
-
-
1.1.1.145 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+ Homo sapiens
-
 androst-5-en-3,17-dione + NADH + H+
-
 ?
1.1.1.145 pregnenolone + NAD(P)+ Homo sapiens
-
 pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
 3beta-HSD I
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 placenta 3beta-HSD I Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.145 0.0091
-
 placental microsomes, 3beta-HSD activity Homo sapiens
1.1.1.145 0.0145
-
 Sf9-cells, 3beta-HSD activity Homo sapiens
1.1.1.145 0.0172
-
 placental microsomes, isomerase activity Homo sapiens
1.1.1.145 0.0257
-
 Sf9-cells, isomerase activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 5alpha-androstan-3beta-ol-17-one + NADP+
-
 Homo sapiens 5alpha-androstan-3,17-dione + NADPH
-
 ?
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
 ?
1.1.1.145 pregnenolone + NAD(P)+
-
 Homo sapiens pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 NAD+
-
 Homo sapiens