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Literature summary extracted from

  • Huang, Y.W.; Lu, M.L.; Qi, H.; Lin, S.X.
    Membrane-bound human 3beta-hydroxysteroid dehydrogenase: overexpression with his-tag using a baculvirus system and single-step purification (2000), Protein Expr. Purif., 18, 169-174.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 overexpression with his6-tag, using a baculovirus expression system Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0017
-
 dehydroepiandrosterone
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.145 microsome
-
 Homo sapiens
-
-
1.1.1.145 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 pregnenolone + NAD(P)+ Homo sapiens
-
 pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
 3beta-HSD I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145
-
 Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.145 0.155
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
 Homo sapiens 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
 ?
1.1.1.145 pregnenolone + NAD(P)+
-
 Homo sapiens pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 NAD+
-
 Homo sapiens