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Literature summary extracted from

  • Rabe, T.; Brandstetter, K.; Kellermann, J.; Runnebaum, B.
    Partial characterization of placental 3beta-hydroxysteroid dehydrogenase (EC 1.1.1.145), delta 4-5isomerase (EC 5.3.3.1) in human term placental mitochondria (1982), J. Steroid Biochem., 17, 427-433.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 Cd2+ 1 mM: strong Homo sapiens
1.1.1.145 Co2+ 1 mM: weak, 100 mM: strong Homo sapiens
1.1.1.145 Cu2+ 1 mM: strong Homo sapiens
1.1.1.145 Fe2+ 1 mM: weak, 100 mM: strong Homo sapiens
1.1.1.145 Mn2+ 1 mM: weak Homo sapiens
1.1.1.145 Ni2+ 1 mM: weak, 100 mM: strong Homo sapiens
1.1.1.145 Zn2+ 1 mM: strong Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.00007
-
 pregnenolone
-
 Homo sapiens
1.1.1.145 0.02
-
 NAD+
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.145 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.145 additional information no effect of Ca2+, Sr2+, Ba2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 pregnenolone + NAD(P)+ Homo sapiens
-
 pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
 Homo sapiens 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
 ?
1.1.1.145 pregnenolone + NAD(P)+
-
 Homo sapiens pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.145 40 45
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.145 20 55
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.145 37
-
 30 min, 50% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.145 9.5
-
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 NAD+ more than 10fold as active as NADP+ Homo sapiens
1.1.1.145 NADP+ 10times less effective than NAD+ Homo sapiens