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Literature summary extracted from

  • Shikita, M.; Talalay, P.
    Preparation of highly purified 3alpha- and 3beta-hydroxysteroid dehydrogenases from Pseudomonas sp (1979), Anal. Biochem., 95, 286-292.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+ Pseudomonas sp.
-
 androst-5-en-3,17-dione + NADH + H+
-
 ?
1.1.1.145 dehydroepiandrosterone + NAD+ Pseudomonas sp. TB
-
 androst-5-en-3,17-dione + NADH + H+
-
 ?
1.1.1.145 pregnenolone + NAD(P)+ Pseudomonas sp.
-
 pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?
1.1.1.145 pregnenolone + NAD(P)+ Pseudomonas sp. TB
-
 pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Pseudomonas sp.
-
-
-
1.1.1.145 Pseudomonas sp.
-
 TB
-
1.1.1.145 Pseudomonas sp. TB
-
 TB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145
-
 Pseudomonas sp.
1.1.1.145 native enzyme to homogeneity by streptomycin and ammonium sulfate precipitation, ion exchange chromatography, gel filtration, and affinity chromatography on an NAD+-linked resin, thereby separation from 3alpha-hydroxysteroid dehydrogenase, EC 1.1.1.50 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.145 88.9
-
-
 Pseudomonas sp.
1.1.1.145 88.9
-
 purified enzyme Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.145 -20°C, 2 mM potassium phosphate buffer, pH 7.0, 50% glycerol, 18 months, 50-70% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
 Pseudomonas sp. 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
 ?
1.1.1.145 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
 Pseudomonas sp. TB 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
 ?
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Pseudomonas sp. androst-5-en-3,17-dione + NADH + H+
-
 ?
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Pseudomonas sp. TB androst-5-en-3,17-dione + NADH + H+
-
 ?
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Pseudomonas sp. ?
-
 r
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Pseudomonas sp. TB ?
-
 r
1.1.1.145 pregnenolone + NAD(P)+
-
 Pseudomonas sp. pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?
1.1.1.145 pregnenolone + NAD(P)+
-
 Pseudomonas sp. TB pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3beta-HSD
-
 Pseudomonas sp.
1.1.1.145 3beta-hydroxysteroid dehydrogenase/isomerase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.145 25
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.145 9
-
 assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 NAD+
-
 Pseudomonas sp.