Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bergerat, A.; Gadelle, D.; Forterre, P.
    Purification of a DNA topoisomerase II from the hyperthermophilic archaeon Sulfolobus shibatae (1994), J. Biol. Chem., 269, 27663-27669.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.2.2 spermidine stimulates Saccharolobus shibatae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 (1S,3S)-3-acetyl-3,5,12-trihydroxy-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 3-amino-2,3,6-trideoxy-alpha-L-lyxo-hexopyranoside
-
 Saccharolobus shibatae
5.6.2.2 4'-(9-acridinylamino)methansulfon-m-anisidide
-
 Saccharolobus shibatae
5.6.2.2 4'-(9-acridinylamino)methansulfon-o-anisidide
-
 Saccharolobus shibatae
5.6.2.2 Amsarcines
-
 Saccharolobus shibatae
5.6.2.2 coumermycin no inhibition Saccharolobus shibatae
5.6.2.2 doxorubicin
-
 Saccharolobus shibatae
5.6.2.2 ellipticines
-
 Saccharolobus shibatae
5.6.2.2 NaCl above 100 mM Saccharolobus shibatae
5.6.2.2 novobiocin no inhibition Saccharolobus shibatae
5.6.2.2 quinolones e.g. CP-115,953 Saccharolobus shibatae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 Mg2+ required Saccharolobus shibatae
5.6.2.2 Mg2+ Mg2+ is effective in the concentration range 1-40 mM, optimal concentration is around 30 mM Saccharolobus shibatae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.2 47000
-
 2 * 47000 + 2 * 60000, SDS-PAGE Saccharolobus shibatae
5.6.2.2 60000
-
 2 * 47000 + 2 * 60000, SDS-PAGE Saccharolobus shibatae
5.6.2.2 230000
-
 gel filtration, sucrose density gradient centrifugation Saccharolobus shibatae

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Saccharolobus shibatae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2
-
 Saccharolobus shibatae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.2.2 additional information
-
-
 Saccharolobus shibatae

Storage Stability

EC Number Storage Stability Organism
5.6.2.2 4°C, 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT, 0.0005% Triton X-100 Saccharolobus shibatae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 additional information changes the linking number of a closed circular molecule by an increment of two Saccharolobus shibatae ?
-
 ?
5.6.2.2 network of DNA rings + ATP + H2O decatenation Saccharolobus shibatae monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 network of DNA rings + ATP + H2O enzyme is more efficient in decatenation than relaxation Saccharolobus shibatae monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O catenation Saccharolobus shibatae catenated DNA networks + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation Saccharolobus shibatae relaxed DNA + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation of negative and positive supercoils Saccharolobus shibatae relaxed DNA + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.2 tetramer 2 * 47000 + 2 * 60000, SDS-PAGE Saccharolobus shibatae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.2 70 80
-
 Saccharolobus shibatae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.6.2.2 50 80 50°C: no activity, 70-80°C: maximal activity Saccharolobus shibatae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.6.2.2 65
-
 pH 7, enzyme concentration 90 ng/ml, less than 15% loss of activity after 40 min Saccharolobus shibatae
5.6.2.2 80
-
 pH 7, enzyme concentration 90 ng/ml, half-life: about 35 min. At an enzyme concentration of 900 ng/ml less than 20% loss of activity after 40 min Saccharolobus shibatae
5.6.2.2 95
-
 pH 7, enzyme concentration 90 ng/ml, more than 90% inactivation after 5 min Saccharolobus shibatae