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Literature summary extracted from

  • Kreuzer, K.N.; Jongeneel, C.V.
    Escherichia coli phage T4 topoisomerase (1983), Methods Enzymol., 100, 144-160.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.2.2 additional information
-
 additional information Km for double-stranded DNA is 0.0015 mg/ml, 0.0045 mM nucleotides Tequatrovirus T4

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 membrane the products of gene 39, MW 57000, and gene 52, MW 48000, are tightly membrane-associated Tequatrovirus T4 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 K+ required Tequatrovirus T4
5.6.2.2 Mg2+ required Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.2 18000
-
 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE Tequatrovirus T4
5.6.2.2 48000
-
 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE Tequatrovirus T4
5.6.2.2 57000
-
 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2
-
 Tequatrovirus T4

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 additional information E. coli, bacteriophage T4-infected Tequatrovirus T4
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.2.2 additional information
-
-
 Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 additional information enzyme also cleaves single-stranded DNA in a site-specific fashion, but this cleavage is mechanistically distinct from the double-strand DNA cleavage Tequatrovirus T4 ?
-
 ?
5.6.2.2 additional information cannot supercoil various E. coli plasmid DNAs in vitro Tequatrovirus T4 ?
-
 ?
5.6.2.2 network of DNA rings + ATP + H2O decatenation Tequatrovirus T4 monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O catenation Tequatrovirus T4 catenated DNA networks + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ?
5.6.2.2 supercoiled DNA + ATP + H2O pBR322 plasmid DNA Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ?

Subunits

EC Number Subunits Comment Organism
5.6.2.2 hexamer 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE Tequatrovirus T4