Literature summary extracted from

Yoshida, T.; Nakata, T.; Ichishima, E.
DNA topoisomerase I from rice: enzyme synthesis in germination, and partial purification from cultured cells (1991), Phytochemistry, 30, 3885-3887.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
5.6.2.1	Berenil	-	Oryza sativa
5.6.2.1	camptothecins	no inhibition	Oryza sativa
5.6.2.1	coumermycin	weak	Oryza sativa
5.6.2.1	Dextran sulfate	-	Oryza sativa
5.6.2.1	diethyl dicarbonate	-	Oryza sativa
5.6.2.1	EDTA	-	Oryza sativa
5.6.2.1	Ethidium bromide	no inhibition	Oryza sativa
5.6.2.1	heparin	-	Oryza sativa
5.6.2.1	Na+	above 100 mM	Oryza sativa
5.6.2.1	novobiocin	weak	Oryza sativa
5.6.2.1	PCMB	-	Oryza sativa

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
5.6.2.1	Na+	maximal activity at 75 mM	Oryza sativa

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
5.6.2.1	80000	-	gel filtration	Oryza sativa

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.6.2.1	Oryza sativa	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
5.6.2.1	partial	Oryza sativa

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
5.6.2.1	seed	-	Oryza sativa	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5.6.2.1	supercoiled DNA	relaxation	Oryza sativa	relaxed closed circular DNA	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.6.2.1	7	-	-	Oryza sativa

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
5.6.2.1	5.5	9.5	50% of maximal activity at pH 5.5 and pH 9.5	Oryza sativa

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Release 2023.1 (January 2023)