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Literature summary extracted from

  • Tricoli, J.V.; Kowalski, D.
    Topoisomerase I from chicken erythrocytes: purification, characterization, and detection by a deoxyribonucleic acid binding (1983), Biochemistry, 22, 2025-2031.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.1 NEM
-
 Gallus gallus
5.6.2.1 single-stranded DNA
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.1 nucleus
-
 Gallus gallus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.1 K+ optimal activity at 270 mM KCl Gallus gallus
5.6.2.1 Mg2+ no requirement for Mg2+ Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.1 68000
-
 sucrose density gradient sedimentation Gallus gallus
5.6.2.1 100000
-
 1 * 100000, SDS-PAGE Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.1
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.1 erythrocyte
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.2.1 additional information
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.1 additional information enzyme is unable to resolve topologically knotted circular duplex DNA Gallus gallus ?
-
 ?
5.6.2.1 supercoiled DNA relaxation Gallus gallus relaxed closed circular DNA
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.1 monomer 1 * 100000, SDS-PAGE Gallus gallus